South West Regional Centre of Excellence for two-dimensional liquid chromatography with high resolution ion mobility mass spectrometry

简单地说，质谱学是一种称重化合物或分子的技术。这是通过中性化合物带电的初始阶段实现的。这被称为电离，一旦电离，这些物种(离子)可以使用磁场和/或电场来分离。对于单个化合物，分析可能很简单，但当处理混合物时，需要其他技术。混合物需要被分离以确定单独的成分；这从根本上是分离科学，有几种不同的方法来进行这项工作。最常见的是层析，这是一种分离多种不同形式的化学混合物的方法。色谱和质谱学的结合为复杂混合物的分析提供了最强大的现代仪器。这项提议将为多进样液相色谱质谱计提供资金。该仪器将提供先进的自动进样技术、复杂混合物的分离和先进的高分辨率质谱仪，以帮助化合物鉴定。进气口将提供无缝集成和自动进样，不受兼容样品类型的限制。液体色谱(单柱)将提供混合物中各组分的分离，但对于高度复杂的混合物，需要二维色谱(双柱)。不同的柱加强了单个化合物的分离，并将分离重叠的化合物。离子迁移率将提供另一种基于形状的分离模式，最后使用高分辨率质量测量来确定化合物的化学式，这允许具有相同标称质量的物种通过其化学式的差异进行分离。尖端样品进样、色谱分离和MS功能的组合将在一系列应用中对复杂混合物和基质中具有战略重要性的新型、重要的极性、热不稳定、不稳定的化合物进行定性和定量分析，这将是英国学术界的一项独特能力。新的战略装备将支持整个南方地区的研究。来自地区合作伙伴(巴斯大学、朴茨茅斯大学、南安普顿大学、萨里大学和斯旺西大学)的科学将发展这一能力，并随后扩展到其他学术和行业研究人员，以形成一个英才中心，使EPSRC能够进行高度优先的研究。

In its simplest terms mass spectrometry is a technique for weighing compounds or molecules. This is achieved through an initial stage where the neutral compound becomes charged. This is called ionisation, and once ionised, these species (ions) can be separated using magnetic and/or electric fields. For individual compounds analysis can be simple, but when dealing with mixtures other technologies are required. The mixtures need to be separated to identify the individual components; this is fundamentally separation science and there are several different ways to undertake this. The most common is called chromatography, which is a method of separating many different forms of chemical mixtures. The combination of chromatography and mass spectrometry affords the most powerful modern-day instrumentation for the analysis of complex mixtures.This proposal will fund a multiple inlets liquid chromatography mass spectrometer. This instrumentation will deliver advanced automated sample introduction techniques, separation of complex mixtures and advanced high resolution mass spectrometry to aid compound identification.The inlets will provide seamless integration and automated sample introduction with no constraints on compatible sample types.Liquid chromatography (one column) will afford separation of components in mixtures but for highly complex mixtures, 2-dimensional chromatography (two columns) are needed. The different columns enhance the separation of individual compounds and will separate of overlapping compounds. Ion mobility will provide another mode of separation based on shape, and finally high resolution mass measurement is used to determine the chemical formulae of compounds, this allows for species with the same nominal mass to be separated by differences in their chemical formulae.The combination of cutting-edge sample inlets, chromatographic separation and MS capability will deliver qualitative and quantitative analysis of novel and strategically important polar, thermally labile, involatile compounds in complex mixtures and matrices across a range of applications and will be a unique capability within the UK academic sector. The new strategic equipment will support research across the Southern region. The science from the regional partners (Universities of Bath, Portsmouth, Southampton, Surrey, and Swansea) will develop the capability and subsequently be extended to other academic and industry researchers to form a Centre of Excellence to enable high-priority EPSRC research.