ANALYSIS OF AUTOCRINE GROWTH OF FOLLICULAR LYMPHOMA CELLS IN VITRO

滤泡性淋巴瘤是一种无痛性疾病，最终导致 迅速增殖的淋巴瘤。调节生长发育的生长因子 滤泡性淋巴瘤细胞的特征没有得到很好的描述。我们已经承诺 找出与此相关的自分泌增长因素(如果有) 用于滤泡性淋巴瘤的体外生长。我们已经完成了初步的 用条件培养液观察滤泡性淋巴瘤细胞 可以用来支持电池在低电镀时的生长 密度。通过这种方式，我们已经能够证明这种独裁 因子由这些细胞产生。无血清粒度分级 条件培养液显示大于10kd的部分是 负责公司的成长促进活动。 条件培养液。卵泡的逆转录/聚合酶链式反应分析 淋巴瘤细胞的信使核糖核酸显示，这些细胞产生几种白介素类， 包括IL-1B、IL-2、GM-CSF和IL-5。IL-1B和IL-2，当加入 细胞在低密度下，促进它们的生长到正常的程度 其在低密度条件下刺激细胞生长的能力是 明显受到抑制。因此，滤泡性淋巴瘤细胞似乎拥有 IL-1B和IL-2的自分泌生长循环。

Follicular lymphomas is an indolent disease which eventually results in a rapidly proliferating lymphoma. Growth factors which regulate the growth of follicular lymphoma cells are not well described. We have undertaken to identify the autocrine growth factors, if any, which are responsible for follicular lymphoma growth in vitro. We have made the initial observation that follicular lymphoma cells with condition medium, which can be used to support their growth when the cells are plated at low density. In this way we have been able to demonstrate that autocrine factors are produced by these cells. Size fractionation of serum-free conditioned medium reveals that a greater then 10 kd fraction is responsible for the growth promoting activity of unfractionated conditioned medium. Reverse transcription/PCR analysis of follicular lymphoma cell mRNA reveals that these cells produce several interleukins, including IL-1B, IL-2, GM-CSF and IL-5. IL-1B and IL-2, when added to cells at low density, potentiate their growth to the sane degree as conditioned medium, its ability to stimulate cell growth at low density is markedly inhibited. Thus, follicular lymphoma cells appear to possess autocrine growth loops for IL-1B and IL-2.