DIAGNOSTIC ELECTRON MICROSCOPY AND CLINICALLY APPLIED RESEARCH

超微结构病理学(UP)部分提供专门的诊断 为难以用常规方法分类的案件提供服务。在……里面 此外，UP部门还长期致力于：提高可用性 低分化肿瘤分类的诊断技术 并确定可能导致以下疾病的预后因素 开发新的治疗策略。因此，这一节 承担过独立研究项目(另有报道) 关于：1)生长因子在分化和分化中的作用 儿童肿瘤的体外增殖；2)肿瘤细胞的鉴定 具有p53突变或mdm基因高表达的亚组可能 预后意义和3)非常规诊断的使用 方法，如逆转录聚合酶链式反应(RT-PCR)进行更多 某些肿瘤的准确特征。 UP组还提供诊断咨询服务， NCI的儿科肿瘤科(POB)，并有合作项目 与POB和临床中心的其他调查人员一起。 由UP部分指导的临床病理项目的例子有1) 尤文肉瘤及外周原始细胞P-糖蛋白的表达 神经外胚层肿瘤(PNET)治疗前后；2)增殖 细胞核抗原是儿童横纹肌肉瘤的预后因素； 3)尤文肉瘤与PNET：组织学对预后有影响吗？ UP部门在其中发挥协作作用的项目示例如下： 1)Ara-C对横纹肌肉瘤细胞系的诱导分化 重组人胰岛素生长因子II在横纹肌肉瘤细胞中的表达 原位杂交；3)治疗后肿瘤消退 抗胰岛素样生长因子II的单抗在横纹肌肉瘤裸鼠模型中的应用 可能诱导分化和/或增加β-2水平- 微球蛋白在难治性神经母细胞瘤合并肿瘤治疗中的应用 浸润性淋巴细胞、干扰素和白介素2；5) 细胞骨架蛋白(微管蛋白和波形蛋白)在血管内皮细胞中的作用 顺铂耐药的发展及紫杉醇诱导的机制 中国仓鼠V79细胞死亡。

The Ultrastructural Pathology (UP) Section provides specialized diagnostic services for cases difficult to classify with conventional methods. In addition, the UP section has a long-term commitment to: improve available diagnostic techniques for the classification of poorly differentiated solid pediatric tumors and to define prognostic factors which may lead to development of new therapeutic strategies. For this reason, the section has undertaken independent research projects (reported separately) regarding: 1) the role of growth factors in the differentiation and proliferation of pediatric tumors in vitro; 2) the identification of subgroups with p53 mutations or high mdm gene expression which may have prognostic significance and 3) the use of non-conventional diagnostic methods, such as the reverse polymerase chain reaction (RT-PCR) for more accurate characterization of certain tumors. The UP section also provides diagnostic consultative services to the Pediatric Oncology Branch (POB) at the NCI and has collaborative projects with both the POB and other investigators in the Clinical Center. Examples of clinicopathologic projects directed by the UP Section are 1) P-glycoprotein expression in Ewing's sarcoma and peripheral primitive neuroectodermal tumor (PNET) before and after treatment; 2) Proliferating cell nuclear antigen as a prognostic factor in childhood rhabdomyosarcoma; 3) Ewing's sarcoma versus PNET: does histology play a prognostic role? Examples of projects in which the UP Section has a collaborative role are: 1) differentiation of rhabdomyosarcoma cell lines with Ara-C; 2) expression of insulin-growth factor (IGF) II in rhabdomyosarcoma cells by in situ hybridization; 3) tumor regression after treatment with a monoclonal anti-IGF II antibody in a rhabdomyosarcoma nude mouse model; 4) possible induction of differentiation and/or increased levels of beta2- microglobulin after treatment of refractory neuroblastoma with tumor infiltrating lymphocytes, interferon gamma and interleukin-2; 5) evaluation of the role of cytoskeletal proteins (tubulin and vimentin) in the development of cisplatin resistance and 6) mechanisms of taxol-induced cell death in Chinese hamster V79 cells.