STRUCTURE-FUNCTION STUDIES OF THE PLATELET-DERIVED GROWTH FACTOR AND RECEPTOR

血小板衍生生长因子(PDGF)是一种强有力的促有丝分裂原。 间充质起源。为了阐明其结构和功能 配体-受体和受体-信号分子的关系 PDGF受体的胞外结构域、胞内结构域 酪氨酸激酶与PI-3同源区2(SH2)和 GAP已经被表达出来。这些蛋白质已被提纯用于结构分析 和功能分析。目前，已经表明N-SH2 PI-3蛋白的结构域通过钙依赖性结合αPDGFR 机制(提交给J Biol Chem的手稿，1993)。更进一步，一个大的 X-射线结晶学和蛋白质组分表达的蛋白质比例 核磁共振波谱技术已经投入使用。目前， 角质形成细胞生长因子(KGF)的三维结构 KGF的一个突变体正在接受分析。分子中有 建立在计算机图形系统上的模型基于三个- 成纤维细胞生长因子的空间结构。

Platelet-derived growth factor (PDGF) is a potent mitogen to cells of mesenchymal origin. In order to elucidate the structure-function relationships between ligand-receptor and receptor-signaling molecules, the extracellular domains of the PDGF receptor, the intracellular tyrosine kinase and the Src-homology region 2 (SH2) of PI-3 kinase and GAP have been expressed. The proteins have been purified for structural and functional analysis. Currently, it has been shown that the N-SH2 domain of PI-3 kinase binds the alphaPDGFR through a calcium dependent mechanism (manuscript submitted to J Biol chem, 1993). further, a large proportion of the proteins expressed for both x-ray crystallography and nuclear magnetic resonance spectroscopy have been committed. Currently, the three-dimensional structure of keratinocyte growth factor (KGF) and a mutant of KGF are in the process of being analyzed. the molecules have been model built on a computer graphics system based on the three- dimensional structure of fibroblast growth factor (FGF).