SIGNAL TRANSDUCTION THROUGH THE ECT2 ONCOGENE PRODUCT

寻找在上皮细胞中表达并拥有 将潜能转化为成纤维细胞使鉴定 新的癌基因ect2。分离的cdna被证明已经被激活。 通过正常产物的氨基末端截断。ECT2蛋白 在255个氨基酸的中心核心内显示出序列相似性 利用断点簇基因bcr的产物，酵母细胞 周期基因、CDC24和DBL癌基因。这些基因中的每个都编码 Rho样小GTP结合的调节分子或效应物 蛋白质。杆状病毒表达的ect2蛋白能够高效地 与Rho和Rac蛋白特异性结合，而DBL蛋白显示 对Rho家族蛋白具有更广泛的结合特异性。因此，ect2 代表不断扩大的家族的新成员，其产品可以 表现出转化特性并与类Rho蛋白相互作用 RAS超级家族。

The search for molecules expressed in epithelial cells and possessing transforming potential to fibroblasts made it possible to identify a novel oncogene, ect2. The isolated cDNA is shown to have been activated by amino terminal truncation of the normal product. The Ect2 protein exhibits sequence similarity within a central core of 255 amino acids with the products of the breakpoint cluster gene, bcr, the yeast cell cycle gene, CDC24 and the dbl oncogene. Each of these genes encodes regulatory molecules or effectors for Rho-like small GTP binding proteins. the baculovirus-expressed Ect2 protein was capable of highly specific binding to Rho and Rac proteins, while the Dbl protein showed broader binding specificity to Rho family proteins. Thus, ect2 represents a new member of an expanding family, whose products can exhibit transforming properties and interact with Rho-like proteins of the Ras superfamily.