DEVELOPMENT OF NANOLITER CAPILLARY LC/MS TECHNIQUES

在质谱分析中的一个长期存在的问题， 和环境样品的绝对水平是分析物 非常低。 解决这个问题的一个方法是开发低容量- 用于质谱仪的高通量传输系统。 我们有 负责开发纳米级封装毛细管的接口 LC和MS。这些毛细管系统提供了相同的优势， 毛细管GC提供了比填充柱GC更宽的LC系统， 分析物进入MS的高通量，但总通量显著降低 需要分析物水平。 目前的事态发展是在以下领域： 纳米级毛细管LC和毛细管区带电泳 同轴连续流快原子轰击MS和电喷雾 电离MS，包括磁扇区上的电喷雾电离 仪器 我们已经将这些技术应用于分离 脂蛋白，如棕榈酰化肺表面活性蛋白SP-C和其他 脂肪酸酰化肽，Edman降解肽，复合物 蛋白质的混合物和青霉素的混合物。

A perennial problem in the mass spectrometric analysis of both biological and environmental samples is that the absolute level of analyte is extremely low. One approach to this problem is to develop low volume- high flux delivery systems for the mass spectrometer. We have undertaken the development of interfaces for nanoscale packed capillary LC and MS. These capillary systems offer the same advantages over wider-bore LC systems that capillary GC offers over packed-column GC, a high flux of analyte into the MS but with a significantly lower total analyte level necessary. Current developments are in the areas of nanoscale capillary LC and capillary zone electrophoresis interfaced with both coaxial continuous flow fast atom bombardment MS and electrospray ionization MS, including electrospray ionization on a magnetic sector instrument. We have applied these techniques to separations of lipoproteins such as palmitoylated lung surfactant protein SP-C and other fatty acid acylated peptides, to Edman degradation peptides, to complex mixtures of proteins and to mixtures of penicillins.