GUANINE NUCLEOTIDE BINDING PROTEINS AS RECEPTOR-EFFECTOR COUPLERS

鸟嘌呤核苷酸结合蛋白（G蛋白）功能的一个家族 在跨膜信号传导中作为受体-效应物耦合剂。 g蛋白 与多种受体结合，包括激素受体， 神经递质、光气味剂和某些生长因子。 执行器 调节功能（积极的，在某些情况下，消极的） G蛋白包括cAMP的形成，磷酸肌醇的分解，钾离子的释放。 和钙离子通道，以及cGMP降解。 我们使用了各种 研究表达、分布、调节、结构的技术 G蛋白的功能。 我们的研究强调了 G蛋白家族 使用肽特异性抗体，我们定义了 G蛋白与受体和效应物偶联的特异性。 我们已经定义了不同的翻译后脂质修饰所必需的 用于G蛋白α和β/γ亚基的膜附着。 我们 在α亚基中产生了突变， 激活，并将其转染到细胞中以确定表型效应 对细胞功能的影响。 这些研究为理解 G蛋白在正常信号转导中作用及其在阐明 G蛋白结构或功能上的可能缺陷， 异常信号转导

A family of guanine nucleotide binding proteins (G-proteins) functions in transmembrane signalling as receptor-effector couplers. G-proteins couple to a diverse array of receptors including those for hormones, neurotransmitters, light odorants, and certain growth factors. Effector functions regulated (positively and, in some instances, negatively) by G-proteins include cAMP formation, phosphoinositide breakdown, potassium and calcium channels, and cGMP degradation. We have used a variety of techniques to study the expression, distribution, regulation, structure and function of G-proteins. Our studies highlight the diversity within the G-protein family. Using peptide specific antibodies, we have defined the specificity of G-proteins in coupling to receptors and effectors. We have defined distinct post-translational lipid modifications necessary for membrane attachment of G protein alpha and beta/gamma subunits. We have created mutations in alpha subunits that cause constitutive activation, and transfected these into cells to define phenotypic effects on cellular function. These studies provide the basis for understanding the role of G-proteins in normal signal transduction and for elucidating possible defects in G-protein structure or function as the basis for abnormal signal transduction.