BRAIN AMINES--REGULATION AND FUNCTION

该项目的主要目标是：(1)检查编队， 脑生物胺及其代谢产物的释放、代谢和处置 给药或毒素诱导的动物模型的变化 人类疾病；(2)确定生物胺在 调节对压力的反应；以及(3)开发可以 适用于人脑生物胺代谢的研究。 在活体内微透析已被用于监测单胺和 它们在不同区域的胞外液中的代谢产物 下丘脑和基底节。受体和转运蛋白 使用来自大脑不同区域的细胞进行了体外检测： 从下丘脑培养的细胞系。 使用微透析的研究结果表明，去甲肾上腺素 (NE)在室旁核(PVN)的释放随年龄的变化而变化 应激源，固定效果最好，低血糖效果最差。 此外，通过单侧阻断去甲肾上腺素能 脑干通路、下丘脑的神经支配程度 可以对原子核进行评估。局部给药甘氨酸，介绍到 微透析探头尖端的区域引起剂量依赖关系， 士的宁~敏感的多巴胺释放。甘氨酸与甘氨酸的相互作用 牛肾上腺髓质膜上的烟碱型乙酰胆碱受体 (体外核磁共振证实)表明，氨基酸调节 该受体位于对士的宁敏感的两个部位之一。 内源性5-羟色胺似乎也影响从 纹状体。

The main objectives of this project are to: (1) examine formation, release, metabolism and disposition of brain biogenic amines and their alterations after administration of drugs or toxin~induced models of human disease; (2) determine the physiologic role of biogenic amines in mediating responses to stress; and (3) develop methods that can be adapted to study of brain biogenic amine metabolism in humans. In vivo microdialysis has been used to monitor levels of monoamines and their metabolites in extracellular fluid in various regions of the hypothalamus and in the basal ganglia. Receptors and trans~porters have been examined in vitro using cells from different regions of brain: in cell lines cultured from the hypothalamus. Results of studies using microdialysis have shown that norepinephrine (NE) release in the paraventricular nucleus (PVN) varies with the stressor, being greatest with immobilization and least with hypoglycemia. Furthermore, by unilateral interruption of ascending noradrenergic pathways in the brain stem, the degree of innervation of the hypothalamic nuclei can be assessed. Locally administered glycine, intro~duced into the regions of the tip of a microdialysis probe, elicits dose~dependent, strychnine~sensitive dopamine release. The interaction of glycine with nicotinic acetylcholine receptors in bovine adrenal medullary membranes (demonstrated in vitro by NMR) suggests that the amino acid modulates this receptor at one of two sites whichare strychnine sensitive. Endogenous serotonin also appears to influence pamine release from the striatum.