CHRONOLOGY OF APPEARANCE OF HCV-RNA AND ANTIBODIES IN HCV INFECTION

丙型肝炎病毒核糖核酸和不同抗体的出现顺序 实验性感染两例丙型肝炎病毒(丙型肝炎病毒)表位 对携带丙型肝炎病毒的黑猩猩进行了研究。这些黑猩猩被注射了 使用来自非反应性捐赠的未经处理的、汇集的血浆 用于抗丙型肝炎病毒。在输液前采集血清样本，然后 输液后每周一次。这些样本进行了抗丙型肝炎病毒检测， 乙肝表面抗原、抗-HBs、抗-HBc、丙氨酸氨基转移酶(ALT)。血清 用聚合酶链式反应检测样本中丙型肝炎病毒核糖核酸的存在。 C100-3、C22-3、C33c和5-1-1抗体的检测 经免疫印迹、RIBA II试验。这两只黑猩猩表现出不同的 丙型肝炎病毒RNA和丙型肝炎病毒的顺序和时间出现的模式 针对不同丙型肝炎病毒表位的抗体。丙型肝炎病毒特异性基因组 一例在输液后一周内在血清中检测到序列 动物和另一只黑猩猩出生9周。ALT值中的高程为 两种动物均于输液后3周观察。

The sequence of appearance of HCV-RNA and of the different antibodies to hepatitis C virus (HCV) epitopes following experimental infection of two chimpanzees with HCV, have been investigated. The chimps were infused with unprocessed, pooled plasma from donations that were non-reactive for anti-HCV. Serum samples were obtained prior to infusion, and then weekly subsequent to infusion. These samples were tested for anti-HCV, HBsAg, anti-HBs, anti-HBc and alanine aminotransferase (ALT). Serum samples were also tested for the presence of HCV-RNA by polymerase chain reaction assay (PCR) and for antibodies to c100-3, C22-3, c33c and 5-1-1 by the immunoblot, RIBA II test. The two chimpanzees showed different patterns in the sequential and temporal appearance of HCV-RNA and of antibodies to the different HCV epitopes. HCV-specific genomic sequences were detected in serum within one week after infusion in one animal and 9 weeks in the other chimp. Elevations in ALT values were observed 3 weeks subsequent to infusion in both animals.