TEMPERATURE AFFECTS O2-CARRYING CAPACITY OF CROSSLINKED HEMOGLOBINS

鉴于hboc在器官灌注中的潜在应用

In view of the potential application of HBOCs in perfusion of organs for cardioplegia during major surgery, or for preservation of donated organs at low temperatures before transplant, we examined temperature dependence profiles of oxygen equilibrium curves (OECs) at 15-37 Oc for a number of human and bovine cross-linked hemoglobins. OECs for these hemoglobins were carried out on the Hemox-Analyzer fitted with a thermostated cell. OECs for modified hemoglobins at 37 Oc were right shifted as compared to unmodified hemoglobin. Lowering the temperature however, resulted in increased affinity towards oxygen. It shifted OECs to the left. However, bovine hemoglobin cross-linked at the beta-beta subunits exhibited significantly different and more favorable oxygenation at very low temperature. Such is the case with a newly developed modified human hemoglobin cross-linked with a pyridoxyl tetraphosphate derivative. This led us to believe that not only the site of modification, but also the nature of the chemical modification plays a crucial role in determining the degree of conformational constrains placed upon the hemoglobin when loading and unloading oxygen. Kinetic characterization of ligand binding to these hemoglobins is now underway using fast reaction techniques in an attempt to relate both equilibrium and kinetic manifestations of hemoglobin function under hypothermic conditions. Part of this work has been published in Biomat. Art. Cell Immob Biotech (1992). A short communication describing this work fully has been prepared for publication.