A COMPARISON OF STRAINS OF E. COLI TO PRODUCE SEPTIC SHOCK IN DOGS

虽然活细菌通常会引起感染， 细菌毒素与活细菌产生的发病率和死亡率， 不知道，如果细菌毒素是重要因素，治疗针对 可能需要这些毒素。 即使没有细菌存活或生长， 这些预先形成的毒素在确定发病率方面可能是重要的， mortality. 针对这些毒素的治疗可能是重要的补充， 抗生素治疗 使用两个E。在犬模型中，我们设计了一项研究， 是生物毒力因子还是细菌毒素更重要 导致败血性休克 血液动力学休克，血培养， 内毒素水平和存活率在两种生物体活的情况下连续进行， 这两种微生物都被杀死，并从这些微生物中纯化内毒素。 本研究大约进行了4人年。 初步结果 这项研究的结果已经在美国临床医学联合会上发表， 研究和重症监护医学协会。 这次演讲赢得了 1990年的海报奖 人们对这些数据很感兴趣， 确定导致感染发病的因素将指导 未来治疗儿童和成人感染性休克的试验。

While live bacteria commonly cause infection, the relative role of bacterial toxins versus live bacteria to produce morbidity and mortality is not known, if bacterial toxins are important factors, therapies directed at these toxins may be required. Even if no bacteria remain alive or grow, these preformed toxins may be important in determining morbidity and mortality. Therapies directed at these toxin may be important additions to antibiotic therapy. Using two E. coli in the canine model, we designed a study to determine whether organism virulence factors or bacterial toxins were more important in producing septic shock. Measures of hemodynamic shock, blood cultures, endotoxin levels and survival were done serially with both organisms live, both organisms killed, and with purified endotoxin from these organisms. Approximately 4 man years have gone into this study. Preliminary results from this study have been presented at the American Federation of Clinical Research and the Society of Critical Care Medicine. This presentation won a poster award in 1990. There has been much interest in these data as determination of the factors that produce morbidity in infection will guide trials for future therapies of children and adults with septic shock.