PRODUCTION OF MONOCYTE COLONY STIMULATING FACTOR BY HUMAN MELANOMA CELLS

在检测人类肿瘤细胞产生各种细胞因子时 株，我们发现测试的6个黑色素瘤细胞系中有6个 结构性产生M-CSF。在测试的5个细胞系中，这一点 产量相对较低(细胞融合时为1-5 ng/ml)。 而其中一株黑色素瘤细胞株RPMI5971释放了大约20- 多出100倍的M-CSF。在几个细胞系中，PMA的添加是 发现M-CSF的产量增加了10倍。此外，我们还有 初步证据表明，当M-CSF存在于足够高的浓度时 浓度，可能会抑制这些细胞的增殖。工作 目前正在研究通过这些细胞产生M-CSF的特性 并确定该细胞因子在黑色素瘤细胞系中是否起作用 调节这些细胞的生长。

While examining the production of various cytokines by human tumor cell lines, we have discovered that 6 of 6 melanoma cell lines tested constitutively produced M-CSF. In 5 of the cell lines tested, this production was relatively low (1-5 ng/ml when the cells were confluent) while 1 of the melanoma cell lines, RPMI 5971, released approximately 20- 100x more M-CSF. In several of the cell lines, the addition of PMA was found to increase M-CSF production 10-fold. In addition, we have preliminary evidence that M-CSF, when present in high enough concentrations, may act to inhibit the proliferation of these cells. Work is currently in progress to characterize the production of M-CSF by these melanoma cell lines and to determine if this cytokine plays a role in regulating the growth of these cells.