LEUKOCYTE COMMON ANTIGEN (CD45) IS INVOLVED IN NEUTROPHIL CHEMOTAXIS

CD45抗原家族是一组高分子量的抗原 所有细胞质膜上表达的糖蛋白 白细胞。CD45具有蛋白酪氨酸磷酸酶活性 特异性调节信号转导和淋巴细胞活化 与T、B淋巴细胞表面受体分子的相关性。然而， CD45在中性粒细胞(PMN)中的功能知之甚少。在这 研究中，中性粒细胞与CD45单抗(MAbs)和 测试它们对四个无关的 化学诱导剂：N-甲酰-甲硫基-亮氨酸苯丙氨酸(FMLP)， 白三烯B4(LTB4)、重组人C5a(C5a)和重组 人中性粒细胞激活蛋白-1(NAP-1)，最近被命名为 白介素8(IL8)。一组CD45单抗，包括IgM单抗，Ahn-12- 1和6个IgG1mAbAhn-12、Ahn-12.2、Ahn-12.3、Ahn-12.4、Hle-1和 KC56(T200)，检测其对中性粒细胞趋化作用的影响。PMN 用两种不同的膜检测方法评价趋化作用：一种检测 对迁移的PMN总数进行量化，另一组测定 处于PMN迁移前沿。Ahn-12.1和KC56(T200)显著 抑制PMN对LTB4和C5a的趋化作用。合同中，Ahn-12和Hle-1 未显著抑制PMN对任何一种细胞的趋化作用 化学诱导剂。CD45单抗均不能抑制中性粒细胞趋化 NAP-1/IL8。这些结果表明PMN CD45表位可能相互作用 与LTB4和C5a受体或受体相关分子和 调节趋化反应。 这项研究的结果发表在1991年2月(哈瓦思，L， Balke，J.A.，Christansen，N.P.，Russell，A.A.，Skubitz，K.M. 部分抗白细胞共同抗原(CD45)抗体抑制人 中性粒细胞趋化性。1991年。J.免疫。146：949-957)。

The CD45 antigen family is a group of high molecular weight glycoproteins that are expressed on the plasma membranes of all leukocytes. CD45 has protein tyrosine phosphatase activity and appears to regulate signal transduction and lymphocyte activation by specific association with receptor molecules on T and B lymphocytes. However, little is known about CD45 function in neutrophils (PMN). In this study, PMN were incubated with CD45 monoclonal antibodies (mAbs) and tested for their chemotactic responses to four unrelated chemoattractants: N-formyl-methionyl-leucylphenylalanine (FMLP), leukotriene B4 (LTB4), recombinant human C5a (C5a), and recombinant human neutrophil activating protein-1 (NAP-1), recently designated as interleukin 8 (IL8). A panel of CD45 mAbs including an IgM mAb, AHN-12- 1, and six IgG1 mAbs, AHN-12, AHN-12.2, AHN-12.3, AHN-12.4, HLe-1, and KC56(T200), were tested for their effects on PMN chemotaxis. PMN chemotaxis was evaluated with two different membrane assays: one assay quantified the total number of migrating PMN and the other assayed the leading front of migrating PMN. AHN-12.1 and KC56(T200) significantly inhibited PMN chemotaxis to LTB4 and C5a. In contract, AHN-12 and HLe-1 did not significantly inhibit PMN chemotaxis to any of the chemoattractants. None of the CD45 mAbs inhibited PMN chemotaxis to NAP-1/IL8. These results indicate that PMN CD45 epitopes may interact with LTB4, and C5a receptors or receptor-associated molecules and regulate chemotactic responses. The results of this study were published in February 1991 (Harvath, l., Balke, J.A., Christiansen, N.P., Russell, A.A., and Skubitz, K.M. Selected antibodies to Leukocyte Common Antigen (CD45) inhibit human neutrophil chemotaxis. 1991. J. Immunol. 146:949-957).