CHARACTERIZATION OF A HUMAN PATHOGENIC MYCOPLASMA FROM HIV INFECTED PATIENTS

该实验室仍在继续研究一种源于支原体的新菌株 从标准NIH-3T3细胞系中分离出DNA 从艾滋病患者的组织中提取。这种被指定为支原体的病原体 隐姓氏杆菌在细胞培养中被发现是有传染性的，在猴子身上被发现是有传染性的 造成浪费综合症，并导致死亡。特定的遗传克隆和抗体 为该试剂获得，并用于探测感染组织。 已经尝试开发分子和血清学分析方法来检测这种病毒 并研究其特异性免疫应答。这些化验方法将用于 评估该病原体的传染性和致病性的意义 在人类身上。通过液体杂交程序进行的遗传分析表明 这种病原体的DNA与发酵剂很相似。M.发酵剂 之前曾被认为与白血病有关。与M. 隐姓埋名增强HIV-1诱导细胞病变效应的能力 体外培养的人T淋巴细胞。

The laboratory continues to work on a new strain of mycoplasma originated from the standard NIH-3T3 cell line after transfection with DNA isolated from AIDS patients' tissues. This agent, designated as Mycoplasma incognitus, was found to be infectious in cell culture, and in monkey to cause a waste syndrome, and death. Specific genetic clones and antibodies were obtained for this agent and was used to probe infected tissues. Attempts have been made to develop molecular and serological assays for the agent and to study the specific immunoresponse. These assays will be used to assess the significance of infectivity and pathogenicity of this agent in humans. Genetic analysis by a liquid hybridization procedure has shown that the DNA of this agent closely resembles M. fermentant. M. fermentant has been associated with leukemia previously. Coinfection with M. incognitus enhances the ability of HIV-1 to induce cytopathic effects on human T lymphocytes in vitro.