MECHANISMS OF PROTEIN FOLDING

目前我们正在研究使用鸡蛋白色的可行性 溶菌酶作为研究双态和非双态的模型蛋白 蛋白质变性，用热或强变性剂诱导， 我们正在进行初步实验，监测 热诱导的溶菌酶解折叠作为pH的函数， 蛋白质差谱和二阶导数光谱 阵列分光光度计有了一个合适的模型系统，最终我们 计划通过平衡和动力学研究蛋白质去折叠/重折叠 技术在两个状态和非两个状态的条件下， 为了理解不同的折叠， 途径。

Currently we are investigating the feasibility of using hen egg white lysozyme as a model protein for studying two-state and non-two-state protein denaturation, induced with heat or with strong denaturants, by varying the pH. We are carrying out preliminary experiments monitoring the thermally induced unfolding of lysozyme as a function of pH by means of protein difference spectra and second derivative spectra with a photodiode array spectrophotometer. With an appropriate model system, eventually we plan to investigate protein unfolding/refolding by equilibrium and kinetic techniques under two-state and non-two-state conditions while utilizing various denaturing agents in an effort to understand different folding pathways.