MOLECULAR GENETIC EVENTS IN LUNG CANCER

答：我们最近报道了心房利钠肽mRNA的表达和 小细胞肺癌肿瘤及肿瘤细胞系的免疫反应性 未产生精氨酸加压素的低钠血症患者(4例)。 肿瘤细胞系的高效液相色谱分析 低钠血症患者的肿瘤及心房肌的mRNA表达 利钠肽揭示了细胞内和细胞外的 多肽似乎是心钠素的28个氨基酸形式， 通常在人体血浆中循环的形式。这些研究是 首次对心钠素的异位生产进行了表征 在小细胞肺癌患者中，可能已经确定了第三个因素 (利钠因子)，已被假设在综合征 不当的抗利尿激素(SIADH)。 我们回顾了234例肺癌患者的临床病程。相比之下， 对于123例非小细胞肺癌(NSCLC)患者中的无一例，18例 16%的小细胞肺癌患者出现低钠血症。这18个人中有10个 有肿瘤细胞系可用，8个表达ANF mRNA，5个表达AVP 10株细胞中有3株同时表达ANF和AVP。所有的10个 细胞株产生ANF和/或AVP mRNA。10种肿瘤细胞的研究 93例无低钠血症的小细胞肺癌患者中有4例发生了ANF MRNA，无一例产生AVP mRNA。从这些研究中，我们观察到 小细胞肺癌患者低钠血症患者的所有肿瘤细胞系均产生 ANF或AVP mRNA，或两者兼而有之。心钠素可能是 之前假设的第三个因素，并在 部分SIADH患者低钠血症的发病机制

A. We have recently reported atrial natriuretic peptide MRNA expression and immunoreactivity in tumor and tumor cell lines from small cell lung cancer patients with hyponatremia who did not produce arginine vasopressin (4). High pressure liquid chromatography (HPLC) analyses of the tumor cell lines and tumors from patients with hyponatremia and MRNA expression of atrial natriuretic peptide have revealed that intracellular and extracellular peptide appears to be the 28 amino acid form of atrial natriuretic peptide, the form that normally circulates in human plasma. These studies are the first to characterize the ectopic production of atrial natriuretic peptide in small cell lung cancer patients and may have identified the third factor (natriuretic factor) that has been hypothesized in the syndrome of inappropriate antidiuretic hormone (SIADH). B. We reviewed the clinical course of 234 lung cancer patients. In contrast to none of the 123 non-small cell lung cancer (NSCLC) patients, 18 of Ill (16%) small cell lung cancer patients had hyponatremia . Ten of these 18 had tumor cell lines available and 8 expressed ANF mRNA, 5 expressed AVP mRNA, and 3 of 10 cell lines produced both ANF and AVP mRNA. All of the 10 cell lines produced ANF mRNA, AVP mRNA, or both. Studies of 10 tumor cell lines from the 93 SCLC patients without hyponatremia showed 4 produced ANF mRNA and none produced AVP mRNA. From these studies we have observed that all tumor cell lines studied from SCLC patients with hyponatremia produce ANF mRNA or AVP mRNA, or both. Atrial natriuretic peptide may be the previously hypothesized third factor and play an important role in the pathogenesis of hyponatremia in some patients with SIADH.