SEQUENCE ORGANIZATION OF CLASS I MHC GENES

这项工作的目的是确定DNA序列的组织 猪主要组织相容性中包含的I类基因 复杂性(SLA)。我们已经确定这个基因家族包含 只有七个成员，其中五个已经被分离出来，他们的DNA 序列已确定。已经确定，该序列 I类SLA基因的组织结构类似于其他 其他物种的I类基因。在家庭内部，这是可能的 根据它们的序列，定义至少三个子家族。 SLA基因之间的同源性在两个基因之间的85%之间 在一个子家庭内到子家庭之间的45%。基因转化 事件似乎引起了复杂的变化，并发生了 在子家族之间。物理映射已经确定了 I类基因定位在500kb以内的单个连锁群上。 CDNA克隆的分离表明只有4个I类基因 在猪的外周血淋巴细胞群体中表达。序列分析 这些克隆已经确定了外显子的位置，并进一步 表明存在多种转录后选择性剪接 事件发生在这些单元格中。

The aim of this work is to determine the DNA sequence organization of class I genes contained in the swine major histocompatibility complex (SLA). We have established that this gene family contains only seven members, of which five have been isolated and their DNA sequences determined. It has been established that the sequence organization of the class I SLA genes is similar to that of other class I genes in other species. Within the family, it is possible to define at least three sub-families, based on their sequences. Homologies between the SLA genes range from 85% between two genes within a sub-family to 45% between sub-families. Gene conversion events appear to give rise to complex alterations, and to occur between sub-families. Physical mapping has established that the class I genes map within 500 kb on a single linkage group. Isolation of cDNA clones has demonstrated that only 4 class I genes are expressed within swine PBL populations. Sequence analysis of these clones has established the locations of exons and further indicates a variety of posttranscriptional alternative splicing events occurs in these cells.