MECHANISM OF TRANSCRIPTIONAL ACTIVATION BY THE HUMAN ETS FAMILY PROTEIN IN VITRO

人类ETS癌基因家族的一个新成员ERG最近被认为是 已确认身份。在ERG兔体内产生一种特异性抗体 从大肠杆菌中表达和纯化的蛋白质。有了抗体，52kD 由ERG基因编码的半衰期为21小时的核蛋白 在人类细胞系中被鉴定。这种蛋白质是在非常 有限的细胞类型；在PMA处理后，它被磷酸化，并且 与PEA3寡核苷酸特异性结合。结果表明， Erg蛋白是特异dna的一个新的ets基因家族成员。 一种结合蛋白，可以调节从细胞膜传递的信号。 进一步鉴定ETS癌基因产物为转录产物 激活剂，这个项目将建立一个体外转录系统， 这将使我们能够研究1)ETS蛋白质和碱性蛋白是如何 转录因子相互作用促进RNA 聚合酶II，以及2)ETS蛋白和其他转录 激活剂协同组织细胞对刺激的反应，例如 生长因子、癌基因等。

A novel member of the human ETS oncogene family, ERG, has recently been identified. A specific antibody was produced in rabbits with erg protein expressed and purified from E. coli. With the antibody, a 52 kD nuclear protein, with a halflife of 21 hours, encoded by the ERG gene was identified in human cell lines. This protein was detected in very limited types of cells; it was phosphorylated after PMA treatment, and bound specifically to PEA3 oligonucleotides. The results suggested that the erg protein is a new ETS gene family member of the specific DNA binding protein which may mediate signals transmitted from the membrane. To further characterize ets oncogene products as transcriptional activators, this project will establish an in vitro transcription system, which will allow us to study 1) how ets proteins and basic transcriptional factors interact with each other to promote RNA polymerase II, and 2) how ets protein and other transcriptional activators cooperate to organize cellular response to stimuli, such as growth factors, oncogenes, etc.