PROMOTION OF MYOCARDIAL ANGIOGENESIS VIA DIRECT APPLICATION OF FGF TO HEART

几种可能导致血管生长的多肽 (血管生成)是在过去几年中被发现的。我们的 最终目的是利用这些药物(S)促进心肌梗死 冠心病患者的血运重建。基本信息 成纤维细胞生长因子(BFGF)就是这样一种多肽，它可能发挥一种 在冠脉侧支形成中起重要作用。我们研究了它的作用。 碱性成纤维细胞生长因子对犬冠状动脉渐进性闭塞侧支血流的影响 左回旋支冠状动脉(Lcx)。在这个实验中，Lcx是 在2至3周内逐渐被无肌样收缩肌闭塞 应用于近端血管，使其灌注区依赖 在抵押品血管上。从无基质植入后10天开始，碱性成纤维细胞生长因子110 每日推注微克(N=8)或生理盐水(N=11) 在阿莫罗德的远侧直接进入LCX。心肌 3天后用放射性标记微球测定血流量 术后每周一次，共5周。侧流是 在显色剂诱导的最大血管扩张过程中被量化，并表示为 缺血区/正常区(IZ/NZ)比值。经过2周的治疗后， 碱性成纤维细胞生长因子处理的狗的IZ/NZ比率超过对照狗；最终 治疗组IZ/新西兰血流比值分别为0.49+/-0.13和0.34+/-0.08 对照组分别为(P&lt；0.05)。我们得出结论，冠状动脉内 碱性成纤维细胞生长因子增强单支冠脉模型犬侧支循环血流量 遮挡。

Several polypeptides with the potential to cause blood vessel growth (angiogenesis) have been identified during the last few years. Our ultimate goal is to utilize these agent(s) to facilitate myocardial revascularization in patients with coronary heart disease. Basic fibroblast growth factor (bFGF) is one such peptide that may play an important role in coronary collateral formation. We studied the effect of bFGF on collateral blood flow in dogs subjected to gradual occlusion of the left circumflex coronary artery (LCX). In this experiment, the LCX was occluded gradually over a 2 to 3 week period by an ameroid constrictor applied to the proximal vessel, rendering its perfusion territory dependent on collateral vessels. Beginning 10 days after ameroid placement, bFGF 110 micrograms (N=8) or saline (N=ll) was given as a daily bolus injection directly into the LCX at a point just distal to the ameroid. Myocardial blood flow was determined with radiolabeled microspheres 3 days after ameroid placement and weekly for 5 weeks thereafter. Collateral flow was quantified during chromonar-induced maximal vasodilation and expressed as an ischemic/normal zone (IZ/NZ) ratio. After 2 weeks of treatment, the IZ/NZ ratio in bFGF treated dogs surpassed that of control dogs; final IZ/NZ blood flow ratios were 0.49+/-0.13 and 0.34+/-0.08 in the treated and control groups, respectively (p<0.05). We conclude that intracoronary bFGF enhances collateral blood flow in this canine model of single coronary occlusion.