ONTOGENY OF THE LUTINIZING HORMONE RELEASING HORMONE SYSTEM

LHRH神经元来源于嗅基板，并迁移到 个脑袋 决定细胞特异性LHRH基因表达的因素以及 LHRH神经元在运动过程中使用的迁移机制 目前尚不清楚。 使用免疫细胞化学和 原位杂交组织化学，我们正在检查产前 LHRH系统的开发。 我们发现细胞外 基质分子纤连蛋白，边界，但不进入迁移 途径，而N-CAM，一种细胞粘附分子， 因此，我们提出LHRH细胞迁移到 大脑使用与N-CAM的正（结合）相互作用， 与纤维连接蛋白的负相互作用，并正在验证这一假设 利用胚胎组织的器官型培养。 转基因小鼠 将人LHRH启动子与SV 40 T抗原融合， 这些动物的生殖功能异常。 没有 中枢神经系统内发现LHRH细胞，但鼻区有肿瘤肿块 检出 从这些肿瘤中，获得稳定的LHRH阳性细胞系。 正在建立。

LHRH neurons are derived from the olfactory placode and migrate into the brain. The factors which dictate cell-specific LHRH gene expression and the migratory mechanism(s) used by the LHRH neurons during their movement into the CNS are currently unknown. Using immunocytochemistry and in situ hybridization histochemistry, we are examining the prenatal development of the LHRH system. We have found that an extracellular matrix molecule fibronectin, borders but does not enter the migratory pathway, whereas N-CAM, a cell adhesion molecule, is abundantly present within it. Hence, we propose the hypothesis that LHRH cells migrate to the brain using a positive (binding) interaction with N-CAM, and a negative interaction with fibronectin, and are testing this hypothesis using organotypic cultures of embryonic tissues. Transgenic mice in which the human LHRH promoter was fused to the SV 40 T-antigen have been generated, and these animals show abnormal reproductive function. No LHRH cells were found within the CNS but tumor masses in the nasal region were detected. From these tumors, a stable LHRH-positive cell line is being established.