HIGH-RESOLUTION HIGH-MASS, TANDEM MASS SPECTROMETER

需要资金购买高质量、高分辨率的 串联式质谱仪。质谱仪将被改装成 商业版的BEQQ仪器。修改 包括八极杆(O)而不是四极杆(Q)，其特征在于 光解离实验的进行及其特点 生物大分子气相化学的基础研究 离子。该仪器将放置在化学系的 对仪器的有效操作和维护感兴趣。 目前由美国国立卫生研究院资助的11名研究人员的研究将得到加强 用这个乐器。此外，其他六名由NIH资助的调查人员 都表达了对在以下方面使用仪器的强烈兴趣 五年了。与埃默里无机化学中心的合作， 最初由Nik资助的也可能提供对 向大学社区以外的研究人员提供了一种工具。 将使用拟议仪器的研究人员都在进行 需要结构的不同领域的面向生物医学的研究 中枢神经系统神经肽、磷酸丝氨酸肽、生物活性的阐明 肽激素，未知的狮身人面像碱基，磷脂，尿毒症生长 抑制物，参与神经激素合成的酶，黄素酶， 和阴离子交换膜蛋白条带3。其他参与 电离辐射对谷胱甘肽代谢的影响 DNA，以及髓过氧化物酶的结构和功能。 其他研究涉及调查两国之间的内在互动 金属离子和生物配体，药物和药物之间的相互作用 蛋白质和主客体分子之间的能量学 生物分子离子的碎裂和基础光化学 这与一些抗艾滋病化合物的密码子有关。这个 建议的工具对这些研究的目标至关重要。 项目。

Funds are requested for the purchase of a high-mass, high-resolution tandem mass spectrometer. The mass spectrometer will be a modified version of a commercially available BEQQ instrument. The modifications include an octapole (o) instead of a quadrupole (q), features for perfomting photodissociation experiments, and features for perfomiing fundamental studies in the gas-phase chemistry of large biomolecular ions. The instrument will be housed in the Chemistiy Department in the interest of eff-icient operation and maintenance of the instrument. The current research of eleven NIH-funded investigators will be enhanced by this instrument. Furthermore, six other NIH-funded investigators have expressed a strong interest in the use of the instrumentation in five years. An association with the Emory Nhcrochemistry Center, originally funded by NIK also may provide an indirect use of the instrument to researchers outside the University community. Researchers who will use the proposed instrument are all performing biomedical-oriented research in diverse fields that require structure elucidation of neuropeptides in CNS, phosphoseryl peptides, bioactive peptide hormones, unknown sphingoid bases, phospholipids, uremic growth inhibitors, enzymes involved in neurohormone synthesis, flavoenzymes, and the anion exchange membrane protein band 3. Others are involved in studying the metabolism of glutathione, effects of ionizing radiation on DNA, and the structure and function of the enzyme myeloperoxidase. Other research involves investigating intrinsic interactions between metal ions and biological ligands, interactions between drugs and proteins and between host and guest molecules, energetics of fragmentations of biomolecular ions, and the fundamental photochemistry that is associated with the acdon of some anti-AIDS compounds. The proposed instrument is essential to the goals of these research projects.