GC/SFC -MS INSTRUMENT : TRIPLE QUADRUPOLE

该应用程序;（i）描述了几个合作的正在进行的研究 依赖于寡糖的详细结构分析， 糖脂和糖蛋白样品;（ii）概述了两个新的艾滋病项目 资助研究HIV和T细胞的结构-功能关系 结合糖蛋白;（iii）总结寡糖测序 正在扩大对协作和工具的要求的方法 时间;和，并列的更大的需要，（四）讨论的限制 由过时的仪器所强加。 最近发表的报告（1-7） 记录过去的合作，这些正在进行的努力需要访问 更现代的仪器和最近开发的方法（8- 10）。 后一种技术结合了联合收割机：（a）改进的分量分辨率和 （B）通过SFC-MS进行分子量鉴定; 与NC 1-敏感性加合物的缀合;和（c）连接和支化 通过高碘酸盐氧化、醛衍生化和SFC-MS获得信息。 这些技术的应用需要负离子聚焦、快速 扫描、SFC-MS和更高质量分析; 1978年的MAT 312 拟议的三重四极杆将： 从ZAB扩散许多问题，释放时间更适合 仪器，（例如，高分辨率、高能CID、MIKES、链接扫描 分析，高质量传输）;（ii）作为更常规的仪器 通过SFC-MS和微孔HPLC-MS进行生物聚合物测序;（iii）维持 随着我们逐步淘汰MAT-312，GC-MS在未来十年的能力;以及， (iv)为进一步发展SFC-MS方法提供了机会， 通过研究低能量碰撞，离子约束， 相关的技术，可以产生更多的结构细节上集中 离子。 除了内部研究和确定的用户群体外， 该设施为医疗区提供常规样本资源， 分析，并作为访问科学家的培训资源，博士后 研究员和研究生在糖缀合物的扩展领域 化学、生物化学和应用质谱。 (Ref. 1010，看 附录）。

This application; (i) describes ongoing research of several collaborating groups that depend on, a detailed structural analysis of oligosaccharide, glycolipid, and glycoprotein samples; (ii) outlines two new AIDS projects funded to study the structure-function relationships of the HIV & T-Cell binding glycoproteins; (iii) summarizes oligosaccharide sequencing methodology that is expanding requests for collaboration and instrument time; and, juxtaposed to the greater need, (iv) discusses the constraints imposed by outmoded instrumentation. Recent published reports (1-7) document past collaborations and these ongoing efforts require access to more contemporary instrumentation and recently developed methodology (8- 10). The latter techniques combine: (a) improved component resolution and molecular wight identification by SFC-MS; (b) enhanced glycan detection by conjugation with an NCI-sensitive adduct; and, (c) linkage and branching information by periodate oxidation, aldehyde derivatization and SFC-MS. Application of these techniques require negative ion focusing, fast scanning, SFC-MS, and higher mass analysis; capabilities not available with our 1978 vintage MAT 312. The proposed triple quadrupole would; (i) diffuse many problems from the ZAB, freeing time more appropriate to that instrument, (e.g., high resolution, high energy CID, MIKES, linked scan analysis, high mass transmission); (ii) serve as a more routine instrument for biopolymer sequencing by SFC-MS and microbore HPLC-MS; (iii) sustain GC-MS capability over the next decade as we phase out the MAT-312; and, (iv) provide an opportunity to further develop SFC-MS methodology for glycan structure by studying low energy collisions, ion confinement, and related techniques that may yield greater structural detail on focused ions. In addition to in-house research, and the user-groups identified, this facility serves the Medical Area as a resource for routine sample analysis, and as a training resource for visiting scientists, postdoctoral fellows, and graduate students in the expanding areas of glycoconjugate chemistry, biochemistry, and applied mass spectrometry. (Ref. 1010, see Appendix).