EXPRESSION OF HEAT-SHOCK GENES IN MOUSE SPERMATOGENIC CELLS

环境胁迫后热休克蛋白（HSP）的合成 是一种高度保守的反应， 不利条件的长期影响。最丰富的蛋白质之一 (P70)生精细胞内的ATP含量与hsp7O密切相关 亲和层析纯化，二维免疫印迹 蛋白质凝胶和肽图谱。P70蛋白是在 精子发生的减数分裂阶段。虽然hsp7O的几个成员 基因家族已被测序并表征其mRNA表达， 对这些基因编码的蛋白质知之甚少。我们开发 P70和hsp70特异性抗血清，以研究其表达和作用 雄性生殖细胞中的这些蛋白质。抗P70的抗血清也被用于 从小鼠精母细胞cDNA文库中选择克隆。其中一个克隆人 与睾丸特异性的、发育调节的3.0 kb mRNA杂交 他的表情不受高温影响。序列分析表明， 该克隆与以前报道的hsp70基因具有高度同源性 家人然而，生精细胞似乎不产生hsp7O mRNA或蛋白质对热应激的反应。这表明P70基因可能与 表达与生精细胞中hsp7O基因的抑制有关 细胞然而，精子发生的过程是异常敏感的， 升高的温度和许多有毒物质和P70可能在 生殖细胞的分化，而不是保护免受 环境压力虽然P70的表达可能有利于肿瘤的发生， 精子发生的过程，这一过程也可能离开男性生殖细胞 特别容易受到环境压力的影响。

The synthesis of heat-shock proteins (hsp) following environmental stress is a highly conserved response and apparently protects cells against long-term effects of adverse conditions. One of the most abundant proteins (P70) in spermatogenic cells is related closely to hsp7O, as shown by ATP affinity chromatography purification, immunoblots of two-dimensional protein gels, and peptide mapping. The P70 protein is synthesized during the meiotic phase of spermatogenesis. Although several members of the hsp7O gene family have been sequenced and their mRNA expression characterized, little is known about the proteins encoded by these genes. We developed antisera specific to P70 and hsp7O to investigate the expression and role of these proteins in male germ cells. The antiserum to P70 was also used to select clones from a mouse spermatocyte cDNA library. One of these clones hybridized to a testes-specific, developmentally regulated 3.0 kb mRNA whose expression was not affected by heat. Sequence analysis indicated that the clone has high homology to previously reported genes of the hsp7O family. However, it appears that spermatogenic cells do not produce hsp7O mRNA or protein in response to heat stress. This suggests that P70 gene expression is linked to suppression of the hsp7O gene in spermatogenic cells. However, the process of Spermatogenesis is unusually sensitive to elevation of temperature and to many toxic agents and P70 may function in germ cell differentiation rather than to protect against the effects of environmental stress. Although P70 expression may Be beneficial to the process of spermatogenesis, this process may also leave male germ cells particularly susceptible to environmental stress.