GERM CELL-SPECIFIC MOLECULES OF SPERMATOZOA

精子生殖细胞特异性分子

• 批准号: 3855970
• 负责人: E M EDDY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3855970
• 关键词: developmental genetics; gene expression; genetically modified animals; germ cells; glyceraldehyde 3 phosphate dehydrogenase; laboratory mouse; meiosis; molecular cloning; regulatory gene; sperm; spermatogenesis

Gene expression during spermatogenesis in mammals is being examined to define intrinsic and extrinsic mechanisms regulating development and function of male gametes. Genes being studied are expressed uniquely in spermatogenic cells and/or during specific stages of their development. They code for germ cell specific isoforms of known proteins and include genes for glycolytic enzymes and proteins involved in cell-cell interaction. For example, cDNAS isolated hybridize with RNAs for glyceraldehyde 3-phosphate dehydrogenase (Gapd-s) and hexokinase expressed only in spermatogenic cells. The Gapd-s gene is expressed after meiosis in haploid spermatids, while expression of the hexokinase gene begins during meiosis in pachytene spermatocytes. Glucose is toxic to isolated spermatids but is required by spermatozoa for in vitro fertilization, indicating that unique regulatory processes occur for glycolysis in spermatogenic cells. In addition, mannose 6-phosphate (M6P) receptors have been identified on spermatogenic cells and shown to bind M6P-bearing ligands produced by Sertoli cells. Multiple transcripts for the cation-dependent M6P receptor in germ cells include 1.2 and 1.4 kb mRNAs not found in somatic cells. They probably arise from alternate polyadenylation signals, one being used in pachytene spermatocytes and the other in spermatids. Furthermore, a cDNA encoding a laminin-binding protein has been isolated and shown to recognize a 1.3 kb transcript in germ cells in addition to the 1.2 kb transcript also seen in somatic cells. Transgenic mice are being produced to examine the regulation of expression of such lineage- and stage- specific genes and the roles of their products. Formerly: Germ Cell-Specific Molecules of Spermatozoa

正在检查哺乳动物精子发生过程中的基因表达 定义调节发展的内在和外在机制 雄配子的功能。 正在研究的基因独特地表达于 生精细胞和/或在其发育的特定阶段。 它们编码已知蛋白质的生殖细胞特异性亚型，包括 细胞间参与糖酵解酶和蛋白质的基因 相互作用。 例如，分离的 cDNAS 与 RNA 杂交 甘油醛 3-磷酸脱氢酶 (Gapd-s) 和己糖激酶表达 仅存在于生精细胞中。 Gapd-s基因在减数分裂后表达 单倍体精子细胞，而己糖激酶基因的表达开始于 粗线期精母细胞的减数分裂。 葡萄糖对分离物有毒 精子细胞，但精子需要进行体外受精， 表明糖酵解发生独特的调节过程 生精细胞。 此外，甘露糖 6-磷酸 (M6P) 受体具有 在生精细胞上被鉴定并显示可结合带有 M6P 的细胞 支持细胞产生的配体。 多个转录本 生殖细胞中的阳离子依赖性 M6P 受体包括 1.2 和 1.4 kb mRNA 体细胞中未发现。 它们可能来自替代 多腺苷酸化信号，一种用于粗线期精母细胞和 其他存在于精子细胞中。 此外，编码层粘连蛋白结合的cDNA 蛋白质已被分离并显示可识别 1.3 kb 的转录物 除了 1.2 kb 转录本外，生殖细胞中也可见于体细胞。 正在生产转基因小鼠以检查表达调节 这些谱系和阶段特异性基因及其产物的作用。 以前：精子生殖细胞特异性分子