EXPRESSION OF HEAT SHOCK GENES IN MOUSE SPERMATOGENIC CELLS

热休克基因在小鼠生精细胞中的表达

• 批准号: 5202263
• 负责人: E M EDDY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5202263
• 关键词: developmental genetics; environmental toxicology; gene expression; gene induction /repression; heat injury; laboratory mouse; meiosis; molecular chaperones; spermatogenesis; stress proteins

Heat shock/stress proteins help to protect cells from increases in temperature and other environmental stresses and serve as chaperones of other proteins. This probably occurs by the heat shock proteins binding to short amino acid recognition sequences in other proteins, thereby causing local conformational changes that restrict additional interactions by such proteins. Spermatogenic cells must be maintained 4-5 degrees below body temperature to develop normally. We are studying the relationship between regulation of expression of Hsp70 heat shock genes in spermatogenic cells and their particular sensitivity to heat and certain environmental agents. The Hsp70 genes are members of one of the four multigene families encoding heat shock proteins. Two of these genes (Hsp70.2 and Hsc70t) are expressed only in spermatogenic cells, where they are regulated developmentally and produce abundant protein. the Hsp70.2 gene is expressed predominantly during the meotic phase of spermatogenesis, while the Hsc70t gene is expressed only during the post- meotic phase. We hypothesize that the proteins encoded by Hsp70.2 and Hsc70t are required by spermatogenic cells to chaperone proteins required for unique meiotic and post-meiotic events, respectively. However, two other genes in this family (Hsp70.1 and Hsp70.3) that are expressed vigorously in response to the shock in most cells, are expressed sparsely in heat-shocked spermatogenic cells. We hypothesize that the limited expression of these genes in spermatogenic cells leaves them particularly sensitive to environmental insults.

热休克/应激蛋白有助于保护细胞免受 温度和其他环境压力，并作为伴侣， 其他蛋白质。这可能是通过热休克蛋白结合 缩短其他蛋白质中的氨基酸识别序列， 导致局部构象变化，限制了额外的 这些蛋白质的相互作用。生精细胞必须保持4-5 体温低于30摄氏度才能正常发育。我们正在研究 Hsp 70热休克基因表达调控与细胞凋亡的关系 生精细胞及其对热的特殊敏感性， 某些环境因素。 热休克蛋白70基因是一个成员， 四个多基因家族编码热休克蛋白。其中两个基因 （Hsp70.2和Hsc 70 t）仅在生精细胞中表达， 它们在发育过程中受到调节并产生丰富的蛋白质。的 热休克蛋白70.2基因主要表达在减数分裂期， Hsc 70 t基因仅在精子发生后表达， 减数分裂期我们推测，Hsp70.2和Hsp70.4编码的蛋白质可能与Hsp70.2和Hsp70.4编码的蛋白质有关。 hsc 70 t是生精细胞所需的伴侣蛋白 分别用于独特的减数分裂和减数分裂后事件。然而两 该家族中表达的其他基因（Hsp70.1和Hsp70.3） 在大多数细胞中，对休克的反应强烈， 在热休克的生精细胞中。我们假设有限的 这些基因在生精细胞中的表达使它们特别 对环境污染敏感。