EXPRESSION OF HEAT SHOCK GENES IN MOUSE SPERMATOGENIC CELLS

热休克/应激蛋白有助于保护细胞免受 温度和其他环境压力，并作为 其他蛋白质。这可能是通过热休克蛋白结合而发生的。 为了缩短其他蛋白质中的氨基酸识别序列，从而 导致局部构象变化，从而限制了 通过这种蛋白质的相互作用。生精细胞必须维持4-5个 低于体温才能正常发育。我们正在研究 热休克基因Hsp70表达调控的关系 生精细胞及其对热和热的特殊敏感性 某些环境保护剂。Hsp70基因是一种 编码热休克蛋白的四个多基因家族。其中两个基因 (Hsp70.2和Hsc70t)仅在生精细胞中表达，其中 它们在发育过程中受到调控，并能产生丰富的蛋白质。这个 Hsp70.2基因主要在水稻减数分裂阶段表达。 精子发生，而Hsc70t基因仅在精子发生后表达 减数分裂阶段。我们假设Hsp70.2和Hsp70.2编码的蛋白质 Hsc70t是生精细胞所需的伴侣蛋白 分别用于独特的减数分裂和减数分裂后事件。然而，有两个 该家族中表达的其他基因(Hsp70.1和Hsp70.3) 在大多数细胞中强烈反应的休克，都是稀疏表达的 在热休克的生精细胞中。我们假设有限的 这些基因在生精细胞中的表达使它们变得特别 对环境的侮辱很敏感。

Heat shock/stress proteins help to protect cells from increases in temperature and other environmental stresses and serve as chaperones of other proteins. This probably occurs by the heat shock proteins binding to short amino acid recognition sequences in other proteins, thereby causing local conformational changes that restrict additional interactions by such proteins. Spermatogenic cells must be maintained 4-5 degrees below body temperature to develop normally. We are studying the relationship between regulation of expression of Hsp70 heat shock genes in spermatogenic cells and their particular sensitivity to heat and certain environmental agents. The Hsp70 genes are members of one of the four multigene families encoding heat shock proteins. Two of these genes (Hsp70.2 and Hsc70t) are expressed only in spermatogenic cells, where they are regulated developmentally and produce abundant protein. the Hsp70.2 gene is expressed predominantly during the meotic phase of spermatogenesis, while the Hsc70t gene is expressed only during the post- meotic phase. We hypothesize that the proteins encoded by Hsp70.2 and Hsc70t are required by spermatogenic cells to chaperone proteins required for unique meiotic and post-meiotic events, respectively. However, two other genes in this family (Hsp70.1 and Hsp70.3) that are expressed vigorously in response to the shock in most cells, are expressed sparsely in heat-shocked spermatogenic cells. We hypothesize that the limited expression of these genes in spermatogenic cells leaves them particularly sensitive to environmental insults.