COLLABORATIVE PROJECTS IN ENVIRONMENTAL HEALTH SCIENCES

环境健康科学领域的合作项目包括 质谱学工作组与其他机构合作的项目 团体，无论是研究所内还是研究所外，都要解决相互之间的问题 利息。这些项目的一个主要焦点是确定 环境、生命系统中的外源生物及其测定 他们的代谢产物。它们的重要性在于， 环境中的化合物与其化合物之间的不利相互作用 与生命系统有关的代谢物是我们健康问题的基础。这些项目 通常涉及复杂混合物的在线分离和鉴定 并经常涉及使用MS中可用的所有乐器技术 实验室包括热喷雾LC/MS(TSP/LC/MS)、FAB/MS和FAB/MS/MS (包括使用连续流动技术)和GC/MS。 一个典型的项目是鉴定H.C.Blue No. 和H.C.Blue No.2。H.C.Blue No.1是一种已知的致癌物质，它与 仅与非致癌的H.C.Blue 2略有不同。代谢 这两种化合物在小鼠体内的分布有很大不同。H.C.蓝色2 观察到产生一种主要的代谢产物。利用GC/MS、HR/MS和 TSP/MS技术，该产品已鉴定为侧链 氧化产物。相比之下，致癌的H.C.Blue 1经历了 脱烷基化为游离胺，从中产生各种代谢物。 游离的芳香胺是已知的致癌物质。因此，区别在于 在致癌行为的基础上可以解释它们的不同 新陈代谢。 本标题所列的其他项目包括分析 牛呼吸道上皮前列腺素、缓激肽的测定 牛奶，肝细胞生长因子的鉴定，以及 用自旋陷阱鉴定自由基产物。

Collaborative projects in the environmental health sciences includes those projects in which the mass spectrometry work-group collaborates with other groups, both within and without the institute to solve problems of mutual interest. A major focus of these projects is the determination of xenobiotics in the environment, in living systems and the determination of their metabolic products. Their importance lies in the fact that the adverse interactions between compounds in the environment and their metabolite with living systems underlie our health concerns. These projects typically involve on-line separation and identification of complex mixtures and often involve use of all instrumental techniques available in the MS lab including thermospray LC/MS (TSP/LC/MS), FAB/MS and FAB/MS/MS (including the use of continuous flow techniques) and GC/MS. A typical project is the identification of the metabolites of H.C. Blue No. 1 and H.C. Blue No. 2. H.C. Blue No. 1 is a known carcinogen which differs only slightly from the non-carcinogenic H.C. Blue No. 2. The metabolic profiles in mice of these two compounds differ significantly. H.C. Blue 2 was observed to yield one major metabolic product. Using GC/MS, HR/MS and TSP/MS technique, this product has been identified as a side chain oxidation product. In contrast, the carcinogenic H.C. Blue 1 undergoes dealkylation to the free amine, from which a variety of metabolites arise. The free aromatic amines are known carcinogens. Therefore, the difference in carcinogenic behavior can be explained on the basis of their different metabolisms. Other projects, which are included in this heading, include analysis of airway epithelium prostaglandins, the determination of bradykinin in bovine milk, the identification of liver cell growth factor, and the identification of the products of free radicals with spin traps.