FMRF-AMIDE LIKE PEPTIDES IN CNS--FUNCTION AND DISTRIBUTION

神经肽Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2(F-8-F-NH2) 最初是由针对软体动物的抗血清检测到的 心脏兴奋性多肽FMRF-NH2及其从牛中的分离 大脑。越来越多的证据表明，F-8-F-NH2可能在 调节阿片类药物的作用。在本研究中，功能角色(S) 包括F-8-F-NH2在内的FMRF-NH2免疫反应(IR)进一步 调查过了。静脉注射F-8-F-NH2抗血清中的免疫球蛋白(作为一种 特定的F-8-F-NH2，拮抗剂)显著减少了 纳洛酮催促吗啡依赖大鼠戒断症状。这 结果和其他研究人员之前的发现，F-8-F-NH可以 催促吗啡依赖大鼠戒断，提示F-8-F-NH2 可能与禁欲综合症有关。脑垂体F-8-F-NH2-IR的研究 表明F-8-F-NH2与Arg(8)之间可能存在相互作用。 加压素。在Brattleboro和盐负荷的老鼠中，已知的动物有 改良AVP生物合成，脑垂体分泌F-8-F-NH2-IR 发现是增加的。此外，在脑下垂体中，出生后 F-8-F-NH2的发育与AVP非常相似。 哺乳动物FMRF-NH2-IR由多种分子形式组成，这些分子形式不是 与FMRF-NH2相同。在这项研究中，增加了3个FMRF-NH2样肽 从大鼠脊髓中分离纯化并鉴定其结构 正在进行中。 本项目的拟议课程是：1)继续研究 F-B-F-NH_2在垂体中的功能作用和2)测定 新分离的FMRF-NH2类化合物的结构和生物活性 多肽。

Neuropeptide, Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (F-8-F-NH2), was initially detected by the antisera raised against the molluscan cardioexcitatory peptide FMRF-NH2 and subsequently isolated from bovine brain. Accumulating evidence suggests that F-8-F-NH2 may have a role in modulating opiate effects. In this study, the functional role(s) of FMRF-NH2 immunoreactivity (IR) including F-8-F-NH2 was further investigated. I.C.V. infusion of IgG from F-8-F-NH2 antiserum (as a specific F-8-F-NH2, antagonist) significantly reduced the number of abstinence signs precipitated by naloxone in morphine-dependent rats. This result and the previous findings of other investigators, that F-8-F-NH, can precipitate withdrawal in morphine dependent rats, suggest that F-8-F-NH2 may be involved in abstinence syndrome. Studies on pituitary F-8-F-NH2-IR indicate that there may be an interaction between F-8-F-NH2 and Arg(8)- vasopressin. In Brattleboro and salt loaded rats, animals known to have modified AVP biosynthesis, secretions of F-8-F-NH2-IR from pituitaries were found to be increased. Furthermore, in the pituitary, the postnatal development of F-8-F-NH2 was found to be very similar to that of AVP. Mammalian FMRF-NH2-IR is composed of multiple molecular forms which are not identical to FMRF-NH2. In this study 3 additional FMRF-NH2-like peptides were purified from rat -spinal cords and determination of their 'structures is in progress. The proposed courses of this project are 1) to continue the study on the functional role of F-B-F-NH, in the pituitary and 2) to determine the structures and biological activities of the newly isolated FMRF-NH2-like peptides.