BIOMEDICAL RESEARCH SUPPORT GRANT

反转录病毒研究中心细胞学实验室要求 购买锚定细胞分析和分类(ACAS)系统的资金， ACAS 570型，由密歇根州奥克莫斯的子午线仪器公司制造。 ACAS系统将允许调查人员分析贴壁细胞 方法类似于现在使用Flow对非贴壁细胞执行的操作 细胞学。ACAS 570将成为流动的伴奏乐器 目前正在使用的细胞仪(Coulter EPICS 753) 实验室。这两种仪器结合在一起，将给研究人员带来更大的 研究正在调查的细胞特征和功能的能力 在他们的个人项目中。ACAS 570有几个可取的 这对流式细胞仪来说是有问题的。最多的 其中明显的一点是它能够分析活的贴壁细胞 可用荧光法测量的参数，不需要单独制备 细胞悬浮液。分离贴壁细胞通常会剥离细胞膜 正是要分析的蛋白质，并干扰细胞到细胞和细胞到 底物相互作用。ACAS 570配备了电机驱动的 工作台、激光光学和荧光检测系统及微型计算机 其控制扫描和分析原位染色的细胞场 使用荧光抗体或其他荧光探针。这台电脑 允许随着时间的推移重复观察相同的细胞。激光 系统可以被编程为自动杀死预定义的细胞，从而 为将来的生长和鉴定而分离的细胞亚群。 能够描述随时间发生的特定变化，并隔离或 分选贴壁细胞是ACAS的独特特征，不能 充分利用了流式细胞术。我们建议使用ACAS 570 在针对表征细胞子集的各种项目中 确定细胞对病毒和病毒蛋白等刺激的反应， 激素、DNA诱变剂、药物、毒素和细胞因子。许多项目 需要ACAS的细胞分离和分选功能。

The Cytometry Laboratory of the Center for Retrovirus Research requests funds to purchase the Anchored Cell Analysis and Sorting (ACAS) system, the ACAS Model 570 manufactured by Meridian Instruments, Okemos, Michigan. The ACAS system will permit investigators to analyze adherent cells in a way similar to what is now performed on nonadherent cells using flow cytometry. The ACAS 570 will be a companion instrument to the flow cytometer (Coulter EPICS 753) presently in service in the Cytometry Laboratory. Together, the two instruments will give researchers greater capability to study cell characteristics and functions under investigation in their individual projects. The ACAS 570 has several desirable capabilities that are problematic for the flow cytometer. The most obvious of these is its ability to analyze live adherent cells for parameters measurable by fluorescence without the need to prepare single cell suspensions. Detaching adherent cells often strips the membranes of the very proteins to be analyzed, and disturbs cell to cell and cell to substratum interactions. The ACAS 570 is equipped with a motor-driven stage, laser optics and fluorescence detection system and micro-computer which controls scaning and analysis of fields of cells stained in situ with fluorescent antibodies or other fluorescent probes. The computer permits repeated observations of the same cells over time. The laser system can be programmed to kill predefined cells automatically allowing subsets of cells to be isolated for future growth and characterization. The ability to characterize specific changes over time and to isolate or sort adherent cells are unique features of the ACAS that cannot be performed adequately using flow cytometry. We propose to use the ACAS 570 in a variety of projects directed at characterizing cell subsets and determining cell responses to stimuli such as virus and virus protein, hormones, DNA mutagens, drugs, toxins and cytokines. Many of the projects require the cell isolation and sorting functions of the ACAS.