FLUOROIMMUNOASSAY APPARATUS

衰减时间常数多的新型稀土络合荧光探针 长于典型有机材料的本底衰减时间常数 提供了极大地提高时间分辨灵敏度的潜力 荧光测定法。然而，如果他们要与放射免疫分析方法竞争， 灵敏度要求变得如此之高，以至于超载恢复 荧光检测器是一个主要的问题。过去在这方面的大量工作 这个问题在很大程度上不适用于非常低的速度和级别要求 在这种情况下。因此，最初的努力正在应用于 确定最佳荧光检测装置和辅助信号 过载限制电路。有关荧光探头的问题，请访问 极低的样品浓度将与 疾控中心对这种方法感兴趣，将其作为艾滋病研究的另一种工具。 最后对激励、光学滤波和 将对数字信号处理进行研究和优化。

New rare earth chelate fluorescent probes with decay time constants much longer than the background decay time-constant of typical organic materials offer the potential of a great improvement in sensitivity of time-resolved fluorimetry. However, if they are to rival radioimmunoassay methods the sensitivity requirement becomes so great that overload recovery of the fluorescence detector is a major problem. Extensive past work on this problem is largely inapplicable to the very low speed and level requirements of this situation. Consequently initial effort is being applied to determining the optimum fluorescence detection device and ancillary signal overload limiting circuitry. Problems related to the fluorescent probe at extremely low sample concentrations will be studied in collaboration with CDC, which is interested in this approach as another tool for AIDS research. Finally an evaluation of the details of excitation, optical filtering, and digital signal processing will be worked out and optimized.