9 4 TESLA WIDE BORE MULTI-NUCLEAR NMR SPECTROMETER

最先进的大口径多核核磁共振仪 在9.4特斯拉的磁场下，一组9个 来自六个不同部门的用户(生理学和生物物理学、 儿科学、医学、生物化学、分子药理学和 发育生物学和癌症)和24个未成年使用者。主修课的 用户，古普塔博士建议对金属离子(Na+，K+，Ca2+， 镁离子)在各种分离的细胞和组织中。他试图 了解金属离子与细胞增殖的关系， 分化、音量调节和激素调控 细胞过程，并研究细胞内的排列紊乱。 大鼠肝脏灌流后离子浓度变化的研究 伴随肝脏再生的代谢变化。约翰·罗森博士 弗兰克·斯图恩将使用大口径核磁共振研究 铅中毒的机制，特别是对细胞的影响 骨细胞中的钙稳态。James Scheuer博士将使用 大鼠心脏灌流多核核磁共振研究心脏的易损性 心脏对心肌缺血，而斯皮策博士将利用 核磁共振检查肾脏磷酸盐转运的临床研究 发展。法布里博士将利用高分辨率核磁共振研究 疟疾寄生红细胞的代谢以及在 结合表面线圈研究镰刀动物模型 细胞血管闭塞。Vern Schramm博士将利用核磁共振技术 催化机理和过渡态结构的阐明 糖水解酶和生物素在丙酮酸中的作用 羧基酶反应。布鲁尔博士将继续追查 核磁共振技术在糖肽和核磁共振研究中的应用 低聚糖与刀豆蛋白A的相互作用 使用L-D以及二维高场质子核磁共振谱 蔗糖酶中复杂碳水化合物结构的阐明 胞吐作用中互补基团突变。所有建议的 主要用户和次要用户将从 大口径多核核磁共振仪器的可用性。

A state-of-the-art wide-bore multinuclear NMR instrument operating at a magnetic field of 9.4 Tesla is needed for a group of 9 major users from six different departments (Physiology & Biophysics, Pediatrics, Medicine, Biochemistry, Molecular Pharmacology and Developmental Biology & Cancer) and 24 minor users. Of the major users, Dr. Gupta proposes NMR studies of metal ions (Na+, K+, Ca2+, Mg2+) in a variety of isolated cells and tissues. He seeks to understand involvement of metal ions in cellular proliferation, differentiation, volume regulation, and hormonal control of various cellular processes, and to study the derangement of intracellular ionic concentrations in perfused rat liver to study ionic and metabolic changes accompanying liver regeneration. Drs. John Rosen and Frank Schanne will use wide-bore NMR for their studies on the mechanism of lead toxicity, especially its effect on cellular calcium homeostasis in bone cells. Dr. James Scheuer will use multinuclear NMR of perfused rat heart to study vulnerability of the heart to myocardial ischemia, while Dr. Spitzer will exploit phosphorous NMR to examine renal phosphate transport during development. Dr. Fabry will utilize high resolution NMR to study metabolism of malaria parasitized red cell as well as, in combination with surface coils, to study an animal model for sickle cell vaso-occlusion. Dr. Vern Schramm will utilize NMR for elucidating the catalytic mechanism and transition state structures of glycohydrolases and the role of biotin in the pyruvate carboxylase reaction. Dr. Brewer will continue to pursue applications of NMR in the study of glycopeptide and oligosaccharide interactions with concanavalin A. Dr. Atkinson uses l-D as well as 2-D high field proton NMR spectroscopy of elucidation of complex carbohydrate structure on invertase complementation groups mutant in exocytosis. All of the proposed major and minor users will derive significant benefit from the availability of a wide-bore multinuclear NMR instrument.