PROTEIN DATABASE WORKSTATION

蛋白质计算机分析的共享资源 通过二维凝胶电泳分离， 要求汇编信息以建立蛋白质数据库。 这一设施将由商业上可获得的软件组成 由Protein Databases，Inc.用于扫描， 定量的二维凝胶和Sun计算机系统， 图形能力。 此资源代表仪器和 该软件是蛋白质分析的前沿， 成为世界上不到12个这样的设施之一。 用户 将能够扫描和分析凝胶中的放射自显影图， 他们的实验室或商业运行，以及银染色 凝胶。 该软件将允许匹配的模式上的 数字化的凝胶图像，数千种蛋白质的定量， 蛋白质数据库的建立和公共数据库的使用。 用户可以访问两个终端，并将输出其数据 通过几种方法，包括数字定量，图表 代表每个蛋白质点的实验变化， 斑点图描绘了蛋白质的合成凝胶图像， 分子量、等电点和数据库编号。 利用这一设施的长期目标是 鉴定涉及细胞特异性、细胞 分化和细胞调节。 此外，该设施 将被用来交流实验中蛋白质的信息 实验，以及从实验室到实验室， 通过使用公共数据库。 协作用户 要求使用此仪器的小组将严重依赖于此 资源，以确定蛋白质参与1）神经元和神经胶质细胞 细胞分化，2）糖皮质激素和生长因子作用， 3)细胞转化和肿瘤发生，4）细胞对 神经毒素，5）造血，6）膜转运系统，和 7)老年痴呆症和衰老。 独特的应用 将这种商业上可获得的软件应用于分析 还提出了超高分辨率巨型2D凝胶。 以来 拟议的设施有能力量化和储存 上千种蛋白质的信息， 促进参与蛋白质鉴定的进展 疾病

A shared resource for the computerized analysis of proteins separated by two-dimensional gel electrophoresis and the compilation of information to build protein databases is requested. This facility would be comprised of commercially available software marketed by Protein Databases, Inc. for the scanning and quantitation of two-dimensional gels and a Sun computer system with graphic capabilities. This resource represents instrumentation and software which is on the forefront of protein analysis and would be one of less than a dozen such facilities in the world. Users will be able to scan and digitize autoradiograms from gels run in their laboratory or run commercially, as well as silver stained gels. The software will allow matching of the patterns on the digitized gel images, quantitation of thousands or proteins, the building of protein databases, and the use of public databases. users will have access to two terminals and will output their data by several means, including numerical quantitation, graphs representing experimental changes in each protein spot and large spot maps depicting a synthetic gel image of proteins located by molecular weight, isoelectric point and data base number. The long-term goals to be addressed using this facility are to identify proteins involved in cell specificity, cell differentiation and cell regulation. In addition, the facility will be used to communicate information on proteins from experiment to experiment, as well as from laboratory to laboratory around the world through the use of public databases. The collaborative user group requesting this instrumentation will rely heavily on this resource to identify proteins involved in 1) neuronal and glial cell differentiation, 2) glucocorticoid and growth factor action, 3) cell transformation and oncogenesis, 4) cell responses to neurotoxins, 5) hematopoiesis, 6) membrane transport systems, and 7) Alzheimer's disease and aging. The unique application of applying this commercially available software to the analysis of ultrahigh-resolution giant 2D gels is also proposed. Since the proposed facility has the capacity to quantify and store information on thousands of proteins, it promises to significantly enhance progress toward the identification of proteins involved in disease.