PEPTIDE SYNTHESIS FACILITY

此应用程序请求为建立共享的 用于合成和表征的仪器设备 多肽。所请求的仪器系统包括 全自动多肽合成仪、氨基酸分析仪和 制备高效液相色谱法用于合成肽的纯化。这个 拟建的设施将为14个主要用户提供服务 一个不同的部门。用户从事各种各样的工作 由美国国立卫生研究院资助的正在进行的项目。 将受益于该设施的项目包括以下研究： 信号肽在生物合成和转运中的作用 蛋白质；血小板与纤维蛋白的生物物理相互作用 纤维蛋白原；作用机理的研究 磷脂酶Al；人类肿瘤的生物学和生物化学- 衍生免疫抑制因子；病毒与病毒的相互作用 H-2抗原；胆固醇转运和脂蛋白代谢； 促黑素细胞激素多肽在血管紧张素转换酶中的作用 心血管系统.低密度的致动脉粥样硬化特性 脂蛋白；黏附介导的轴突生长引导； 神经肽分泌的控制；催产素的中枢控制 慢性乙醇处理对细胞分泌的影响 血管平滑肌蛋白质合成的神经内分泌调节； 螺旋体的微生物学和致病机制；以及酶 参与中性粒细胞的代谢反应 中性粒细胞。拟议中的设施将增强能力 加快这些项目的进展速度。

This application requests funding for the establishment of a shared instrumentation facility for the synthesis and characterization of polypeptides. The requested instrument systems include an automated peptide synthesizer, an amino acid analyzer, and a Preparative HPLC for purification of synthetic peptides. The proposed facility would provide services for 14 major users from a different departments. The users are engaged in a wide variety of ongoing projects funded by the National Institutes of Health. Projects that would benefit from the facility include studies of: the role of signal peptidase in the biosynthesis and transport of proteins; biophysical interactions of platelets with fibrin and fibrinogen; investigations of the mechanism of action of phospholipase Al; the biology and biochemistry of a human tumor- derived immunosuppressive factor; the interactions of viral and H-2 antigens; cholesterol transport and lipoprotein metabolism; the role of gamma melanocyte stimulating hormone peptides in the cardiovascular system; atherogenic properties of low density lipoproteins; adhesion-mediated guidance of axonal growth; the control of neuropeptide secretion; the central control of oxytocin secretion; the effects of chronic ethanol treatment on the neuroendocrine regulation of protein synthesis in smooth muscle; the microbiology and pathogenesis of spirochetes; and enzymes involved in the metabolic responses of polymorphonuclear neutrophils. The proposed facility would enhance the capabilities of and accelerate the rate of progress on each of these projects.