DEVELOPMENT AND EVALUATION OF A NEW CULTURE MEDIUM FOR YEAST

免疫功能低下的宿主，包括那些患有艾滋病或 或癌症，容易受到这两种细菌引起的感染 和真菌。 最常见的真菌感染是由酵母菌引起的 如假丝酵母属、球拟酵母属或隐球菌属。 虽然这些 微生物在简单的培养基上生长良好，如血液，巧克力，或 沙氏琼脂，这些媒体不允许容易识别 当可能存在两种或三种不同酵母的混合物时。 这些琼脂上的菌落形态也不能容易地用于 提示可能存在哪些酵母菌。 为了 便于从患者体内分离和鉴定酵母菌 标本，我们开发了一种培养基，产生良好的酵母生长 同时也有助于区分不同种类的酵母 根据菌落形态和颜色，新培养基将 在与沙氏琼脂的比较中进行评价， 用于真菌培养的种植培养基。 此外，媒体将 用作额外的常规血液培养基， 加强血液中酵母菌的检测。

Immunocompromised hosts, including those patients with either AIDS or cancer, are predisposed to infections caused by both bacteria and fungi. The most common fungal infections are caused by yeast such as Candida, Torulopsis or Cryptococcus. Although these organisms grow well on simple media such as blood, chocolate, or Sabouraud's agars, these media do not permit easy recognition of when a mixture of two or three different yeast may be present. Colony morphologies on these agars also cannot be readily used to suggest which species of yeast may be present. In order to facilitate isolation and identification of yeast from patient specimens, we developed a medium which yields good growth of yeast but which also helps differentiate the different species of yeast on the basis of colony morphology and color, The new medium will be evaluated in a comparison with Sabouraud's agar as a primary planting medium for fungal cultures. In addition, the medium will be used as an additional routine blood culture medium in order to enhance the detection of yeast in blood.