RADIOLYSIS, PHOTOLYSIS AND SONOLYSIS OF CELLS AND THEIR CONSTITUENTS

抗肿瘤药物阿霉素，柔红霉素， 某些喹啉盐，和gilvocarcin V进行了研究， 与可能的联合化疗和 光动力疗法 通过使用电子自旋共振和 自旋捕获技术，和（17-0）富集水和氧， 超氧自由基、羟基 自由基和单线态氧的光化学，这些 抗肿瘤药物。 超声波的化学效应 研究了与高热（与 放射治疗）以及诊断和 治疗应用。 温度变化的影响 在不同的稀有气体存在下的空化气泡， 自由基的形成和剪切应力引起的 测量了超声波对哺乳动物细胞的作用。 羟基自由基 产率按雅阁顺序为氙>氪>氩>氖>氦 空化气泡的温度较高。 然而，在这方面， 剪切应力诱导的哺乳动物细胞裂解与 所有稀有气体和独立于它们的热导率， 空化气泡的温度。 产生的自由基 在核酸和蛋白质组分的水溶液中， 超声已经通过自旋捕获被识别，并与 那些由电离辐射和紫外线光解形成的， 过氧化氢的存在。 观察到的同位素交换 反应表明，可能使用声化学方法， 合成氘代蛋白质和核酸成分。

The photochemistry of the antitumor drugs adriamycin, daunomycin, certain quinolinium salts, and gilvocarcin V was investigated in relation to the possible combination of chemotherapy with photodynamic therapy. By the use of electron spin resonance and spin trapping techniques, and of (17-0) enriched water and oxygen, the relative contributions of superoxide radicals, hydroxyl radicals, and singlet oxygen to the photochemistry of these antitumor drugs was elucidated. The chemical effects of ultrasound were studied in relation to hyperthermia (used in combination with radiation therapy) and the possible consequences of diagnostic and therapeutic applications. The effect of varying the temperature of cavitation bubbles in the presence of different rare gases on free radical formation and the shearing stress induced by ultrasound on mammalian cells was measured. The hydroxyl radical yields were in the order xenon>kryton>argon>neon>helium in accord with the higher temperatures of the cavitation bubbles. However, mammalian cell lysis induced by shearing stress was the same for all rare gases and independent of their thermal conductivity and the temperature of the cavitation bubbles. The radicals generated in aqueous solutions of nucleic acid and protein components by ultrasound have been identified by spin trapping and compared with those formed by ionizing radiation and by UV-photolysis in the presence of hydrogen peroxide. The observed isotopic exchange reactions suggest the possible use of sonochemical methods for the synthesis of deuterated protein and nucleic acid constituents.