ETS ONCOGENE EXPRESSION IN BACTERIA AND YEAST

C-ets癌基因最初是通过其病毒 禽急性白血病病毒E26的v-ets基因。这 核蛋白的癌基因编码，许多针对它的 已经提出了一些克隆抗体。这些抗体被用于 检测ETS蛋白在细菌中的表达(E.Coliw3110) 和酵母(S288C单倍体)细胞。针对该病毒的抗体 在酵母细胞中发现了56kD的Ets-2蛋白，如 在作为阳性的Colo 320双分钟(DM)细胞中也是如此 对照，但在细菌细胞中未能做到这一点。此外, 经检测，一种新的单抗呈阳性。 在酵母和人类Colo 320 DM细胞中都有信号。

The c-ets oncogene was originally discovered through its viral equivalent v-ets gene of the avian acute leukemia virus, E26. This oncogene codes for nuclear proteins against which a number of monoclonal antibodies have been raised. These antibodies were used to check for ets protein expression in bacterial (E. coli w3110) and yeast (S288C haploid) cells. The antibody directed against the 56 Kd ets-2 protein was found to give a signal in yeast cells, as well as in the COLO 320 double-minute (DM) cells used as a positive control, but failed to do so in the bacterial cells. In addition, a new monoclonal antibody tested was found to give a positive signal in both the yeast and the human COLO 320 DM cells.