GERM CELL-SPECIFIC MOLECULES OF SPERMATOZOA

哺乳动物精子发生的发育过程导致 在结构和功能上高度分化的细胞的形成。 目标是了解内在和外在的机制。 调节这些细胞的功能和特定阶段的 生殖细胞特异性蛋白基因的表达。它是 假设这些蛋白质负责独特的 精子和精子的结构和功能特征 生精细胞。我们正在使用单克隆抗体来 鉴定、定位和分离这些蛋白质，并将使用 重组DNA方法克隆和鉴定他们的基因。 精子鞭毛的细胞骨架蛋白已被鉴定 似乎是生殖细胞特异的中间丝(IF) 蛋白质。其中一些蛋白质是在精母细胞中合成的， 而另一些则首先出现在精子细胞中。他们是公认的 由抗体产生的IF蛋白还具有体细胞的独特性 抗原性和生化特性。这些蛋白质似乎是 时间调控基因的产物仅在 精子发生。其他研究也在考察 体细胞对生殖细胞功能和基因表达的影响。离体 研究表明趋化性参与了细胞的迁移。 原始生殖细胞(PGC)至生殖脊。它也是 发现一种酶和一种抗原从它的表面丢失 在他们进入山脊后，PGC。此外，精子 必须与附睾部相互作用才能受精。 在这个过程中，精子表面会被糖蛋白修饰。 由附睾上皮分泌。这些糖蛋白是 被分离并鉴定它们在精子中的作用 成熟。相关研究涉及到一种新的纯化方法 Sertoli细胞产品，稳定其中一些的结合 附睾糖蛋白进入精子表面直到接近时间 受精的问题。

The developmental process of spermatogenesis in mammals results in formation of cells highly differentiated in structure and function. The goals are to understand the intrinsic and extrinsic mechanisms that regulate the function of these cells and the stage-specific expression of genes for germ cell-specific proteins. It is hypothesized that such proteins are responsible for unique structural and functional characteristics of sperm and spermatogenic cells. We are using monoclonal antibodies to identify, localize, and isolate such proteins, and will use recombinant DNA methods to clone and characterize their genes. Cytoskeletal proteins of the sperm flagellum have been identified that appear to be germ cell-specific intermediate filament (IF) proteins. Some of these proteins are synthesized in spermatocytes, while others are first present in spermatids. They are recognized by antibodies to IF proteins of somatic cells but also have unique antigenic and biochemical properties. These proteins appear to be products of temporally regulated genes expressed only in spermatogenesis. Other studies are examining the influence of somatic cells on germ cell function and gene expression. In vitro studies have shown that chemotaxis is involved in migration of primordial germ cells (PGC) to the-genital ridges. It was also found that an enzyme and an antigen are lost from the surface of PGC after they enter the ridges. In addition, the spermatozoon must interact with the epididymis to gain the ability to fertilize. During this process, the sperm surface is modified by glycoproteins secreted by the epididymal epithelium. These glycoproteins are being isolated and characterized to identify their roles in sperm maturation. Related studies are concerned with purification of a Sertoli cell product which stabilizes binding of some of these epididymal glycoproteins to the sperm surface until near the time of fertilization.