KINETICS, REGULATION AND MECHANISMS OF BIOCHEMICAL REACTIONS

(1)产品代谢物的直接转移机制 通过多酶复合体将酶转化为利用酶 建议用于糖酵解途径。鉴于它的基本原理 重要的是，我们重新调查了直接转移 α-甘油-3-磷酸与其络合物之间的NADH 脱氢酶(GPDH)和乳酸脱氢酶(LDH)。我们的 结果显示，拟议的NADH在 GPDH和LDH主要是基于对 实验数据，尽管对他们的数据进行了一些微小的修正 也是必要的。(2)解释……影响的理论 环境中的振荡和能量驱动的波动 影响酶催化活性的参数，特别是 那些嵌在细胞膜中的物质已经被配制出来。这个 蛋白质吸收外界振荡能量的能力 参数对其构象自由度的影响 强调。该理论为能源提供了一种合理的机制 转导，即使转导是从动态的 时间平均为零的微扰。此外，一个 正在建造振荡式电脉冲装置，用于 目的是对上述理论进行实验验证。(3) 亲和纯化的抗泛素和抗泛素-BSA结合物 已经准备好并表征了来自绵羊的。这些抗体 用来探测内源性泛素和泛素蛋白 各种组织中的结合物，用于免疫细胞化学 脑部薄片的分析。(4)净化和 兔泛素活化酶的特性研究 网织红细胞和人红细胞，以及异肽酶的 进步。(5)Fe(II)(亚铁氮)3的反应动力学和机理 研究了Fe(II)(氨基酸)3的形成。明智的一步 提出了反应机理，并指定了反应速率常数。(6)型号 关于确定约束性的理论和实践的研究 使用作业图变化法进行化学计量 使用Fe(II)-亚铁锌体系。结果显示，对于 协同结合系统，异常的化学计量比可以是 获得。

(1) A direct transfer mechanism for metabolites from the producing enzyme to the utilizing one via multienzyme complexes has been proposed for the glycolytic pathway. In view of its fundamental importance, we reinvestigated the evidence for direct transfer of NADH between its complexes with alpha-glycerol-3-phosphate dehydrogenase (GPDH) and with lactate dehydrogenase (LDH). Our results revealed that the proposed direct transfer of NADH between GPDH and LDH is mainly based on a misinterpretation of the experimental data, although some minor corrections of their data are also necessary. (2) A theory for explaining the effect of oscillations and energy-driven fluctuations on the environmental parameters on the catalytic activities of enzymes, particularly those embedded within cell membranes, has been formulated. The ability of proteins to absorb energy from oscillating external parameters into their conformational degree of freedom was emphasized. The theory provides a reasonable mechanism for energy transduction, even when the transduction is derived from a dynamic perturbation with a time average of zero. In addition, an oscillating electric pulse apparatus is being constructed for the purpose of experimentally verifying the above theory. (3) Affinity-purified anti-ubiquitin and anti-ubiquitin-BSA conjugates from sheep have been prepared and characterized. These antibodies were used to probe endogenous ubiquitin and ubiquitin-protein conjugates in various tissues, and used for immunocytochemical analysis of thin sections of brain. (4) Purification and characterization of ubiquitin activating enzyme from rabbit reticulocytes and human erythrocytes, and of isopeptidase are in progress. (5) Kinetics and mechanism of Fe(II)(ferrozine)3 formation from Fe(II)(amino acid)3 was investigated. A step wise mechanism was proposed and rate constants assigned. (6) Model study to examine the theory and practice for determining binding stoichiometry using the Job plot variation method was carried out using the Fe(II)-ferrozine system. The results revealed that for cooperative binding systems, anomalous stoichiometries can be obtained.