CLONING OF THE CDNA FOR AVIAN INTESTINAL EPITHELIAL BRUSH BORDER CALDESMON

Caldesmon是一种细丝钙调蛋白结合蛋白 似乎在肌动蛋白-肌球蛋白的调节中起作用 在平滑肌和非肌肉细胞中的相互作用。有在 发现至少两种钙调蛋白亚型，一种高分子量形式 主要存在于平滑肌和一种较低分子量的形式 主要存在于非肌肉细胞中。我们正在尝试克隆和测序 在鸡肠刷边发现的钙离子。一旦这一次 我们想要使用细菌表达载体 并对表达的蛋白质和基因工程进行了研究 变种。

Caldesmon is a thin filament calmodulin-binding protein which appears to play a role in the regulation of actin-myosin interaction in smooth muscle and nonmuscle cells. There are at least 2 isoforms of caldesmon, a high molecular weight form found primarily in smooth muscle and a lower molecular weight form found mainly in nonmuscle cells. We are attempting to clone and sequence the caldesmon found in chicken intestinal brush border. Once this is accomplished we would like to use bacterial expression vectors and study the expressed protein as well as genetically engineered variants.