IDENTIFICATION AND ISOLATION OF C-FMS PROTOONCOGEN FROM F344/N RAT LEUKEMIA

原癌基因c-fms在两种成熟的猫科动物中均有发现。 巨噬细胞和未分化的小鼠和人肿瘤，以及 正常人骨髓细胞 c-fms原癌基因是 在人单核细胞分化过程中表达， 该产品与单核细胞受体相关， 吞噬细胞生长因子（CSF-1）。 本研究的目的是：（1）筛选 c-fms原癌基因在体内连续移植的 F344/N单核白血病细胞系，（2）启动研究 基因产物的分离和表达。 这将 提供对肿瘤起源的了解，并帮助提供 肿瘤的诊断标准。 此外，这种肿瘤细胞系可能 为研究提供了宝贵的遗传物质来源 c-fms原癌基因及其 对MNCL表达的潜在影响。 初步调查 初步结论表明可能存在c-fms 从体内移植的MNCL肿瘤系中分离的癌基因。

The proto-oncogene, c-fms, has been described in both mature cat macrophages and undifferentiated mouse and human tumors, and normal human bone marrow cells. The c-fms proto-oncogene is expressed during human monocyte differentiation and the c-fms product has been related to the receptor for mononuclear phagocyte growth factor (CSF-1). The purpose of this investigation is: (1) to screen for the presence of the c-fms proto-oncogene in the in vivo serially transplanted F344/N mononuclear leukemic cell lines and, (2) to initiate studies on the isolation and expression of the gene product. This will provide insight on the origin of the tumor and aid in providing diagnostic criteria for the tumor. In addition, this tumor line may provide a valuable source of genetic material for investigations into the molecular biology of the c-fms protooncogene and its potential effect on MNCL expression. Preliminary investigation and tentative conclusions indicate the possible presence of c-fms oncogene isolated from in vivo transplanted MNCL tumor lines.