ELEMENTAL MAPPING OF TRYPANOSOMA CRUZI BY ANALYTICAL ELECTRON MICROSCOPY

对两个不同的无性系进行了元素分析 克氏锥虫(一种引起查加斯病的致病原虫) 疾病)，以前被证明在 它们的毒性和光学特性。分析结果是 通过BEIB中的元素映射实现 计算机化分析电子显微镜。样本是 通过将整个细胞快速冷冻在氨水中制备而成 硝酸盐缓冲液，支撑在Forvar/Carbon EM格栅上； 在分析之前，样品是冷冻替代品。X射线图 铁、锌、镁、钾、钙、磷和S是从一些 每个克隆的上胚体。不同的铁含量 为两种细胞类型建立的，其中一个克隆具有 铁含量大约是另一种的两倍，大多数 看起来像在线粒体区域，可能 归因于细胞色素。其他不同之处在于 二价阳离子(镁、钙和锌)的含量，主要是 与磷酸盐结合在一起的致密小体散布在 细胞。

Elemental analyses were obtained from two different clones of Trypanosoma Cruzi (a pathogenic protozoan causing Chagas' disease) that had previously been shown to have differences in their virulence and optical properties. The analyses was accomplished by means of elemental mapping in BEIB's computerized analytical electron microscope. Samples were prepared by rapidly freezing whole cells placed in an ammonium nitrate buffer and supported on formvar/carbon EM grids; samples were freeze-substitutes prior to analysis. X-ray maps of Fe, Zn, Mg, K, Ca, P, and S were recorded from a number of epimastigotes from each clone. Different iron contents were established for the two cell types, one clone having approximately twice the Fe content of the other, with most appearing to be in the mitochondrial region and possibly attributable to cytochromes. Other differences were seen in the amounts of divalent cations (Mg, Ca and Zn), which were mainly bound with phosphate in dense bodies scattered throughout the cells.