ACQUISTITION OF HIGH FIELD NMR SPECTROMETER

核磁共振的最新进展已经导致了新一代的 光谱仪的实验能力大大提高。 这一点，沿着已经被充分证明的优势， 场核磁共振研究生物系统，将进一步提高 核磁共振在解决生物学结构问题方面的贡献。 随着大量的序列信息， 最近使用分子生物学的新技术产生的， 用实验方法来理解分子 构象将使我们进入下一个目标，发展结构- 功能关系，以便主要功能的 可以确定序列数据。 在本提案中， 要求支付购买高场的部分费用 核磁共振光谱仪。 这个光谱仪将大大改善 埃默里大学的核磁共振设施，并将提供更好的访问 到核磁共振仪器的工作人员在生物化学和 物理系没有这样的仪器， 自己的. 对这一工具的研究的一个主要重点是 是进一步开发新方法的研究重点 仪器将进一步开发新的灵敏度方法 利用质子探测的异质性增强异质核 多量子相干实验 这些实验将 提供来自质子以外的原子核的新信息， 有助于分析复杂的生物大分子。 的 仪器将支持总共12项研究 来自埃默里大学的9名调查人员， 化学，物理和生物化学各一名， 来自格鲁吉亚州立大学的调查人员。 讨论的研究 这里涵盖了广泛的生物医学相关系统 包括激素，合成疫苗，药物与DNA的相互作用， 酶和膜相互作用。

Recent advances in NMR have lead to a new generation of spectrometers with greatly increased experimental capabilities. This, along with the already well documented advantages of high field NMR in studying biological systems, will further enhance the contribution of NMR in solving structural problems in biology. With the large amount of sequence information that has been generated recently using new techniques in molecular biology this experimental approach to understanding the role of molecular conformation will move us to the next goal, developing structure- function relationships so that the full implications of primary sequence data can be determined. In this proposal, funds are requested to cover part of the cost of purchasing a high field NMR spectrometer. this spectrometer would greatly improve NMR facilities at Emory University and will provide better access to NMR instrumentation for workers in the Biochemistry and Physics Departments which do not have such instrumentation of their own. A major focus of the research on this instrument will be to further develop new methods focus of the research on this instrument will be to further develop new methods for sensitivity enhancement of heteronuclei using proton detected heteronuclear multiquantum coherence experiments. These experiments will make available new information from nuclei other than protons to aid in the analysis of complex biomacromolecules. The instrument will support research of a total of twelve investigators, nine investigators from Emory University, seven in Chemistry and one each in Physics and Biochemistry, and three investigators from Georgia State University. Research discussed here covers a wide range of biomedically related systems including hormones, synthetic vaccines, drug-DNA interactions, enzymes, and membrane interactions.