ACQUISTITION OF HIGH FIELD NMR SPECTROMETER

核磁共振的最新进展导致了新一代核磁共振 大大提高了光谱仪的实验能力。 这一点，以及已经很好地记录下来的高 场核磁共振在生物系统研究中的应用，将进一步提高 核磁共振在解决生物学结构问题中的贡献。 随着大量的序列信息被 最近利用分子生物学的新技术产生的 理解分子作用的实验方法 构象将把我们推向下一个目标，发展结构- 功能关系，以便充分体现初级 可以确定序列数据。在这项提案中，资金是 要求支付购买高地油田的部分成本 核磁共振波谱仪。这台光谱仪将大大改进 埃默里大学的核磁共振设施，将提供更好的访问 为生物化学和核磁共振工作者提供核磁共振仪器 没有这样的仪器的物理系 他们自己的。该仪器研究的一个主要焦点将是 是进一步发展新方法研究的重点 仪器将进一步开发新的灵敏度方法 用质子探测的异核增强异核 多量子相干实验。这些实验将 将质子以外的原子核的新信息提供给 协助分析复杂的生物大分子。这个 仪器将支持总共12项研究 调查人员，9名来自埃默里大学的调查人员，7名 化学，物理和生物化学各一门，三门 佐治亚州立大学的调查人员。讨论的研究 这里涵盖了一系列与生物医学相关的系统 包括激素，合成疫苗，药物与DNA的相互作用， 酶和膜相互作用。

Recent advances in NMR have lead to a new generation of spectrometers with greatly increased experimental capabilities. This, along with the already well documented advantages of high field NMR in studying biological systems, will further enhance the contribution of NMR in solving structural problems in biology. With the large amount of sequence information that has been generated recently using new techniques in molecular biology this experimental approach to understanding the role of molecular conformation will move us to the next goal, developing structure- function relationships so that the full implications of primary sequence data can be determined. In this proposal, funds are requested to cover part of the cost of purchasing a high field NMR spectrometer. this spectrometer would greatly improve NMR facilities at Emory University and will provide better access to NMR instrumentation for workers in the Biochemistry and Physics Departments which do not have such instrumentation of their own. A major focus of the research on this instrument will be to further develop new methods focus of the research on this instrument will be to further develop new methods for sensitivity enhancement of heteronuclei using proton detected heteronuclear multiquantum coherence experiments. These experiments will make available new information from nuclei other than protons to aid in the analysis of complex biomacromolecules. The instrument will support research of a total of twelve investigators, nine investigators from Emory University, seven in Chemistry and one each in Physics and Biochemistry, and three investigators from Georgia State University. Research discussed here covers a wide range of biomedically related systems including hormones, synthetic vaccines, drug-DNA interactions, enzymes, and membrane interactions.