ONCOGENIC ACTIVATION OF EGF RECEPTOR AND RELATED GENES

基因正常编码生长因子受体的机制 可以被“激活”，因为致癌基因正在研究中。该基因的分子克隆 禽红母细胞增殖症病毒ES4分离株V-erb基因 被构建成小鼠逆转录病毒载体(MuLV)，一种病毒已经 将此DNA导入NIH/3T3细胞后获得 “获救”的是一种辅助白血病病毒。这种新的小鼠假型 V-erb基因现在被用于研究它是如何改变生长的。 几种体外系统的性质及分化程序 鼠源的。 分子结构已经被工程化为逆转录病毒载体 测试最近分离的HER-2基因的转化潜力(也 称为neu)。蛋白质的氨基酸截断“激活”了它的 转化能力；双氨基和羧基截断不能 增强后一种效果(如在其他系统中所建议的)。病毒库 如上所述从HER-2的这个转化对应物获得 该基因目前正在进行体内和体外测试。

The mechanisms by which genes normally encoding for growth factor receptors can be "activated" as oncogenes is being studied. A molecular clone of the v-erb gene from the ES4 isolate of avian erythroblastosis virus (AEV) has been constructed into a murine retroviral vector (MuLV) and a virus has been obtained after transfection of this DNA into NIH/3T3 cells and "rescued" with a helper leukemogenic virus. This new murine pseudotype of the v-erb gene is now being used to study how it alters the growth properties and the differentiation program of a number of in vitro systems of murine orgin. Molecular constructions have been engineered into retroviral vectors to test the transforming potential of the recently isolated HER 2 gene (also called neu). The amino truncation of the protein "activates" its transforming ability; a double amino and carboxy truncation does not enhance this latter effect (as suggested in other systems). Virus stocks obtained as described above from this transforming counterpart of the HER 2 gene are now being tested in vivo and in vitro.