STUDIES ON THE ACTIVATION OF ONC GENES IN VIRUSES AND HUMAN TUMORS

我们检测到了两个可转化的转化序列 人卵巢癌细胞系OVCAR3 DNA的筛选 裸鼠移植瘤实验。形态可分辨变换 活动对糖皮质激素地塞米松的反应不同 (DEX)，它取消了我们检测形态变换的能力 由两个序列中的一个诱导的。我们克隆了一个保守的alu+ 该DEX敏感转化子的10.5kb片段。它代表了 两个通常不相连的序列的融合，显然发生在 转染法。 我们构建了一种携带禽类Gag的小鼠逆转录病毒(ME26)，如 以及来自禽红白血病的v-myb和v-ets序列 病毒，E26。当通过复制拯救病毒时，病毒会复制到高滴度 小鼠白血病病毒，感染该病毒的细胞表达一个140-kD 与抗禽GAG和抗ETS抗血清反应的融合蛋白。这个 病毒似乎不能转化NIH3T3小鼠胚胎成纤维细胞。 我们已经将一种基于牛乳头瘤病毒(BPV)的构建物 含有人类金属硫蛋白基因和小鼠肉瘤病毒mos 基因，要么与人生长激素(HGH)序列在 金属硫蛋白控制，或直接与金属硫蛋白启动子融合。 这两种构建物似乎都能转化小鼠C127细胞和NIH3T3细胞。 在存在的情况下，可以检测到非常高水平的mos融合蛋白。 Cd仅存在于HGH融合构建体转化的细胞中。

We have detected two transfectable transforming sequences following the screening of DNA from a human ovarian carcinoma cell line, OVCAR3, in the nude mouse tumor assay. The morphologically distinguishable transforming activities show differential response to the glucocorticoid dexamethasone (DEX) which abolishes our ability to detect morphological transformation induced by one of the two sequences. We have cloned a conserved alu + 10.5-kb fragment from this DEX-sensitive transformant. It represents the fusion of two normally unlinked sequences which apparently occurred during transfection. We have constructed a murine retrovirus (ME26) which carries avian gag, as well as v-myb and v-ets sequences derived from the avian erythroleukemia virus, E26. The virus replicates to high titer when rescued by replicating murine leukemia virus, and cells infected with the virus express a 140-Kd fusion protein which reacts with anti-avian gag and anti-ets antisera. The virus does not appear to transform NIH3T3 mouse embryo fibroblasts. We have transfected a bovine papillomavirus (BPV)-based construct which contains the human metallothionein gene and the murine sarcoma virus mos gene, either fused to human growth hormone (HGH) sequences under metallothionein control, or fused directly to a metallothionein promoter. Both constructs appears to transform mouse C127 cells and NIH3T3 cells. Very high levels of mos fusion protein can be detected in the presence of cadmium only in cells transformed by the HGH fusion construct.