GERM CELL-SPECIFIC MOLECULES OF SPERMATOZOA

针对精子、生精细胞或 分离的抗原正被用来鉴定和表征分子 特定于生殖细胞。正在测试的一般假设是细菌 细胞特异性基因产物负责独特的结构和 精子的功能特征。纤维鞘是一种 位于质膜下面和周围的细胞骨架结构 哺乳动物主要部分的外密纤维和轴丝 精子鞭毛。一种能识别一种蛋白的单抗被发现 2DSDS-PAGE和Western blotting检测表观MR 66.7K和pI 8.5 程序。对精子发生过程中的抗原表现进行了分析 从幼年和成年小鼠中分离出生殖细胞。66.7K抗原首先出现 出现在已发现的精子细胞中。然而，一个被识别的78K抗原 相同的抗体和存在于精母细胞发育的整个过程消失 在圆形精子细胞和细长精子细胞阶段之间。使用其他 抗体表明这是由于抗原表位的修饰，而不是 降解，并且这种蛋白质也被结合到 纤维鞘。目前正在测试的假设是，纤维状的 鞘蛋白是一种生殖细胞特异的中间丝蛋白。 精子表面成分的研究涉及一种31K糖蛋白 出现在附睾成熟过程中。已经使用了一种单抗 以显示抗原出现在附睾体的血浆上 鞭毛的膜。这种抗原是在体外从精子中排出的 受精条件但保留在附睾液存在的情况下。 从附睾液中提纯抗原稳定因子 从Sertoli细胞条件培养液中研究分子的性质 以及它在附睾成熟和获能过程中的作用。

Monoclonal antibodies prepared to spermatozoa, spermatogenic cells, or isolated antigens are being used to identify and characterize molecules specific to germ cells. The general hypothesis being tested is that germ cell-specific gene products are responsible for the unique structural and functional characteristics of spermatozoa. The fibrous sheath is a cytoskeletal structure which underlies the plasma membrane and surrounds the outer dense fibers and axoneme in the principal piece of the mammalian sperm flagellum. A monoclonal antibody was found to recognize a protein of apparent Mr 66.7 K and pI 8.5 using 2D SDS-PAGE and Western blotting procedures. Antigen appearance during spermatogenesis was analyzed using germ cells isolated from juvenile and adult mice. The 66.7 K antigen first appears in found spermatids. However, a 78 K antigen recognized by the same antibody and present throughout spermatocyte development disappears between the round spermatid and elongating spermatid stages. Use of other antibodies indicates that this is due to epitope modification rather than degradation and that this protein also becomes incorporated into the fibrous sheath. The hypothesis currently being tested is that the fibrous sheath protein is a germ cell-specific intermediate filament protein. Studies on sperm surface components involve a 31 K glycoprotein that appears during epididymal maturation. A monoclonal antibody has been used to show that the antigen appears in the corpus epididymidis on the plasma membrane of the flagellum. This antigen is shed from sperm under in vitro fertilization conditions but retained in the presence of epididymal fluid. The antigen stabilizing factor is being purified from epididymal fluid and from Sertoli cell conditioned medium to study the nature of the molecule and its role in epididymal maturation and capacitation.