SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES

口腔的健康是靠唾液分泌维持的。这个 唾液腺的主要功能是产生这些复杂的液体。 我们主要利用体外分散的细胞和膜制剂 大鼠唾液腺以了解控制唾液形成的机制。 我们将这些研究的重点放在自主神经递质的调节上。 分泌事件和相关的信号机制。老龄化的大鼠 腮腺已被用作研究自主神经的有用模型。 外分泌腺泡细胞中钙离子处理的受体控制。在此期间 本报告所述期间，有关这些准备工作的具体研究领域包括 (1)腮腺基底外侧的ATP依赖性钙泵特性 膜和(2)α-肾上腺素受体动员的机制 细胞内的钙离子。我们还将我们的努力扩大到开发和 利用细胞培养研究外分泌事件。为此，我们 已经(1)开始描述与以下相关的功能性事件 在三个已建立的上皮细胞系中的分泌，以及(2)启动 几种唾液腺上皮细胞的原代培养。

The health of the oral cavity is maintained by salivary secretions. The principal function of salivary glands is to produce these complex fluids. We utilize primarily in vitro dispersed cell and membrane preparations of rat salivary glands to understand mechanisms controlling saliva formation. We have focused these studies on autonomic neurotransmitter regulation of secretory events and associated signaling mechanisms. The aging rat parotid gland has been employed as a useful model to study autonomic receptor control of Ca2+ handling in exocrine acinar cells. During this reporting period specific areas of study with these preparations include (1) characteristics of the ATP-dependent Ca2+ pump in parotid basolateral membranes and (2) mechanistic aspects of Alpha-adrenoreceptor mobilization of cellular Ca2+. We have also expanded our efforts into developing and utilizing cell culture to study exocrine secretory events. To this end we have (1) begun to characterize functional events, associated with secretion, in three established epithelial cell lines and (2) initiated primary cultures of epithelial cells from several types of salivary glands.