A RESOURCE FOR BIOMEDICAL MASS SPECTROMETRY

华盛顿大学质谱学资源计划研究： 1.为当地、地区和地区提供广泛的低分辨率GCMS服务 国内和国际生物医学研究人员 2.提供专家技术援助、分析服务和 对高精度GCMS选择离子监测的解释建议 底物含量的定量(SIM-GCMS)和稳定同位素富集法 在生理样本中。 3.提供全自动同位素比质谱仪(IRMS)，用于 用于稳定同位素测量的快速、高进样量操作 内源底物过期和排出的最终产物的浓缩 新陈代谢。 4.建立了高同位素比值监测GCMS(IRM-GCMS) 小样本量、高精度、高精度的稳定同位素分析 样品制备比IRMS更少，并具有测量能力 比SIM-GCMS更大的示踪剂稀释度。 5.建立高分辨率质谱学--快原子电离 用于分析高分子量或热不稳定物质的设备 分子。 6.研制高效液相色谱-热喷雾质谱仪 多肽测序的常规方法及其他高效液相色谱-质谱仪的建立 分析。 7.使新开发的设施可供符合条件的 地方、地区、国家和国际各级的调查人员。

Research at Washington University Mass Spectrometry Resource plans: 1. to provide wide range low resolution GCMS service to local, regional, national, and international biomedical investigators 2. to provide expert technical assistance, analytical services, and interpretive advice on high precision GCMS selected ion monitoring quantitation (SIM-GCMS) of substrate content and stable isotope enrichment in physiologic samples. 3. To provide a fully automated isotope ratio mass spectrometer (IRMS) for rapid, high sample volume operation for measurement of stable isotope enrichment in expired and excreted end products of endogenous substrate metabolism. 4. To develop an isotope ratio monitoring GCMS (IRM-GCMS) for high precision quantitative stable isotope analysis with smaller sample size and less sample preparation than with IRMS and with the ability to measure greater tracer dilution than possible with SIM-GCMS. 5. To establish a high resolution mass spectrometry-fast atom ionization facility for the analysis of high molecular weight or thermally labile molecules. 6. To develop a facility for HPLC-thermospray mass spectrometry to augment conventional methods for peptide sequencing and establish other HPLC-MS analyses. 7. To make the newly developed facilities available for use by qualified investigators at the local, regional, national, and international levels.