CHARACTERIZATION OF OPIOID AND PEPTIDE RECEPTORS IN BRAIN AND PERIPHERAL TISSUES

我们已经使用定量的配体结合研究来表征 大鼠阿片和其他多肽受体的多种类型和亚型 脑、牛肾上腺髓质、牛垂体后叶等 纸巾。我们已经证明了催产素受体的存在和一个 V1亚型加压素受体在猪睾丸、排卵细胞、细胞膜中的分布 白膜、附睾和输精管。一种新型的加压素 在普鲁丁精囊中发现了浓度较高的受体 超过肾髓质中的那些。与V1受体不同，这些受体 受体与腺苷环化酶偶联。牛神经分泌体 脑下垂体后叶含有高浓度的阿片类药物 受体，专属于“Kappa”类型：Mu、Delta和 不存在苯并吗样点。至少两类网站 不同的亲和力(Kd约为1 NM，Kd约为1um)为 现在时。 哇巴因、地高辛和洋地黄毒素在大鼠脑和心脏中的受体 肌肉的特征也由两类部位组成， 两者对K+都很敏感。脂肪酸类物质对人体健康的影响 这些心脏糖苷和阿片肽的结合已被研究。 系统地。

We have used quantitative ligand binding studies to characterize the multiple types and subtypes of opioid and other peptide receptors in rat brain, bovine adrenal medulla, bovine posterior pituitary, and other tissues. We have demonstrated the presence of receptors for oxytocin and a V1 subtype of vesopressin receptor in porcine testis, leyding cells, tunica albuginea, epididymis and vas deferens. A new, novel type of vasopressin receptor has been demonstrated in procine seminal vesicle at concentrations in excess of those in renal medulla. Unlike the V1 receptor, these receptors are coupled to adenylate cyclase. Neurosecretosomes from bovine posterior pituitary are shown to contain high concentrations of opioid receptors, which are exclusively of the "Kappa" type: Mu, delta and benzomorphan sites are not present. At least two classes of sites of differing affinities (Kd approximately 1 nM, Kd approximately 1 MuM) are present. Receptors for ouabain, digoxin, and digitoxin in rat brain and cardiac muscle have been characterized, and also consist of two classes of sites, both of which are sensitive to K+. The effects of fatty acids on the binding of these cardiac glycosides and of opioid peptides has been studied systematically.