PHE-MET-ARE-PHE-NH2 LIKE PEPTIDES IN THE BRAIN AND SPINAL CORD

我们之前已经分离出了两种推定的 内源性阿片拮抗剂多肽。在这项研究中，针对 这两种多肽的产生及其在体内的分布 用放射免疫分析法检测脑和脊髓，并 免疫组织化学技术。进行免疫组织化学研究。 在加州大学Pertti Panula博士的合作下完成 芬兰赫尔辛基。这两种多肽被发现是不均匀的。 分布于脑内，以脊髓背部浓度最高 脊髓和中脑导水管周围灰质区，皮质和 小脑。在脊髓中，在板层发现有免疫反应。 后角的I-II。在免疫组织化学方面，有几组 大鼠脑内神经元呈免疫反应；免疫反应细胞 发现于大脑皮层、外侧隔核的斜角带， 新纹状体、下丘脑室周区和几个延髓 原子核。 两种内源性阿片受体拮抗剂的鉴定 脑神经元中的多肽表明这两种多肽可能有 在神经传递中起作用。这两种多肽在体内的富集度 脊髓背侧和中脑导水管周围灰质区与我们的过去 研究结果提示，这两种多肽可能参与疼痛。 调制。 两种推定的内源性阿片类药物的研究方向 拮抗肽是：(1)研究其机制。 抗阿片作用(2)确定受体的分布和(3) 探讨这些多肽在阿片类药物开发中的可能作用 宽容。

We have previously isolated and chemically characterized two putative endogenous opiate antagonist peptides. In this study, antibodies against these two peptides were produced and distribution of these two peptides in brain and spinal cord was investigated by radioimmunoassay and also immunohistochemical technique. The immunohistochemical study was carried out with the cooperation of Dr. Pertti Panula at the University of Helsinki, Finland. These two peptides were found to be unevenly distributed in the brain with the highest concentrations in dorsal spinal cord and periaqueductal gray area and the lowest levels in cortex and cerebellum. In the spinal cord, immunoreactivities were found in laminae I-II of the posterior horn. Immunohistochemically, several groups of neurons in the rat brain were immunoreactive; immunoreactive cells were found in the cerebral cortex, lateral septal nucleus of the diagonal band, neostriatum, periventricular hypothalamic areas and several medullary nuclei. The identification of the two putative endogenous opiate antagonist peptides in brain neurons suggest that these two peptides may have functions in neurotransmission. The enrichment of these two peptides in dorsal spinal cord and periaqueductal gray area together with our past findings led us to suggest that these two peptides may participate in pain modulation. The proposed courses of the study on the two putative endogenous opiate antagonist peptides are: (1) to investigate the mechanism underlying the antiopiate action (2) to determine the distribution of receptors and (3) to explore the possible role of these peptides in development of opioid tolerance.