PURIFICATION OF MESSENGER RNAS ENCODING FOR NEUROTROPIC FACTORS IN RAT BRAIN

神经营养因子已被证明出现在大鼠脑后， 损伤。 本项目拟从分子水平克隆编码 大脑中这些损伤诱导的营养因子。 1985年，我们开发了产生这些因子的测定方法， 它们的神经营养活性 一部分mRNA，从受损的 大鼠脑，负责神经营养活性，发现在体内 在非洲爪蟾卵母细胞中翻译成蛋白质。 在过去的几个月里，我们构建了一个特异于 在Bluescribe MB载体中的损伤的大鼠脑。 的选择 阳性克隆和相应基因的测序正在进行中。

Neurotrophic factors have been shown to appear in the rat brain after lesion. This project intends the molecular cloning of the gene encoding for these lesion-induced trophic factors in the brain. During 1985, we developed the assays to produce these factors and test for their neurotrophic activity. A fraction of mRNA, prepared from lesioned rat brain, responsible for neurotrophic activity was found by in vivo translation into proteins in Xenopus Laevis oocytes. During the last past months, we constructed a cDNA library specific for lesioned rat brain in the Bluescribe MB vector. The selection of the positive clones and the sequencing of the corresponding gene is in progress.