GENE REGULATION WITHIN THE NERVOUS SYSTEM

我们研究了细胞特异性和发育调节的表达， 神经系统内的蛋白质使用神经元特异性（NSE）， 非神经元（NNE）烯醇化酶同工酶作为模型。 人脑cDNA和 构建了基因组DNA文库， 可以分离和表征其它脑特异性蛋白质。 使用 抗体和寡核苷酸探针，人NSE和 NNE已被分离和测序。 利用这些独特的区域 cDNA克隆作为探针，发育和细胞特异性调节 这些蛋白质中的每一种的mRNA的出现可以使用 原位交联。 这些基因的人类染色体位点 将鉴定同工酶。 此外，分离人类基因组 这些蛋白质中每一种的克隆应该提供关于 细胞周期中神经元和胶质细胞特异性蛋白表达的调控 人类神经系统在正常和疾病状态下的分化。 NSE对神经源性细胞系的正常特异性和 NSE特异性DNA探针的可用性将提供有用的 神经源性正常细胞和肿瘤细胞的鉴定方法 血统

We approached the cell-specific and developmentally regulated expression of proteins within the nervous system using the neuron specific (NSE) and non-neuronal (NNE) enolase isozymes as a model. Human brain cDNA and genomic DNA libraries were constructed so that the genes for these and other brain specific proteins could be isolated and characterized. Using both antibodies and oligonucleotide probes, cDNAs for both human NSE and NNE have been isolated and sequenced. Employing unique regions of these cDNA clones as probes, the developmentally and cell-specific regulated appearance of mRNA for each of these proteins can be investigated using in-situ hydridization. The human chromosome loci for each of these isozymes will be identified. In addition, the isolation of human genomic clones for each of these proteins should provide information on the regulation of expression of neuron and glial specific proteins during cell differentiation of the human nervous system in normal and disease states. The normal specificity of NSE for neural derived cell lines and the availability of specific DNA probes for NSE should provide a useful approach to the characterization of neural derived normal and tumor cell lineages.